| Project/Area Number |
03670508
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
NAGAKURA Toshikazu Department of Pediatrics, Associate Professor, 医学部, 助教授 (30125025)
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| Co-Investigator(Kenkyū-buntansha) |
MASAKI Takuro Department of Pediatrics, Lecturerer, 医学部, 講師 (00119851)
坂口 直哉 東京慈恵会医科大学, 医学部, 助手 (80215623)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Gas Chromatography Mass Spectrometry / Prostaglandin / Thromboxane / Exercise-induced asthma / 肺機能 / 運動誘発喘息 / TXB_2 / PGE_2 / PGF_<2α> / 6ーketoーPGF_<1α> |
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| Research Abstract |
he purpose of this study is to clarify the role of prostaglandins (PGs) and thromboxanes (TXs) in the pathogenesis of exercise-induced asthma (EIA). PGs and TXs were measured by Gas Chromatography Mass Spectrometry (GC/MS) using selective ion monitering (SIM). The reason of using GC/MS instead of radio immuno assay (RIA) to measure eicosanoids in human plasma and urine is that the specificity obtained by GC/MS is superior than that of RIA.
Exericise test was performed by cycle ergometer to achieve a workload of 80% of maximal oxygen consumption. Venous blood or urine was collected before and after exericise.
_<9alpha> - _<11beta> PGF_2 which is a relatively stable metabolite of PGD_2 elevated in the subjects with EIA but not regative EIA subjects. TXB_2 which is one of the stable metabolited of TXA_2 did not changed in EIA.Orally administerd OKY-046 which is a TX synthetase inhibitor blocked EIA.The baseline urinary out put of TXB_2 significantly decreased after one-week administration of OKY^2-046. Plasma levels of PGF_<2alpa> and 6 keto PGE_1 elavated in EIA.
Our results suggest that bronchoconstrictive eicosanoids such as _<9alpha 11beta> PGF_2, PGF_<2alpha> and TXA_2 might be involved in the pathogenesis od EIA.
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