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Transmembrane Signalling System in Psoriasis

Research Project

Project/Area Number 03670519
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionAsahikawa Medical College

Principal Investigator

IIZUKA Hajime  M.D.Asahikawa Medical College, Department of Dermatology, Professor and Chairman, 医学部, 教授 (90113513)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hidetoshi  M.D.Asahikawa Medical College, Department of Dermatology, Assistant, 医学部, 助手 (00216748)
TSUTSUI Masato  M.D.Asahikawa Medical College, Department of Dermatology, Instructor, 医学部, 講師 (10172032)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Keywordspsoriasis / protein kinase C / involucrin / retinoid / keratinization / cyclic AMP / TPA / プロテイン キナーゼ / 表皮 / アデニル酸ミクラ-ゼ / βアドレナリン受容体 / グルココルチコイド / プロテインキナ-ゼC / 脱感作 / G蛋白質
Research Abstract

1) Psoriatic involved epidermis was characterized by defective beta-adrenergic adenylate cyclase response. This was observed by the treatment of keratinocytes with protein kinase C activators as well as by the treatment of cells with the beta-adrenergic agonists.
2) Glucocorticoids augment the beta-adrenergic adenylate cyclase response of keratinocytes through the induction of the beta2-adrenergic receptor.
3) Protein kinase C activators induced the expression of involucrin, that is one of the major constituent of keratinocyte cornified envelope. The induction of involucrin by the protein kinase C activators was jun/fos-dependent process and was inhibited by glucocorticoids.
4) Retinoid, Ro 10-1670, augmented the beta-adrenergic adenylate cyclase response in pig skin organ culture system. However, the retinoid decreased the beta-adrenergic adenylate cyclase response in FRSK culture system. In the latter system the decreased beta-adrenergic response was accompanied by the increased thymidine incorporation of FRSK cells.
5) Superoxide dismutase activity was markedly decreased in the psoriatic involved epidermis. This was due to the decreased Cu, Zn-SOD activity. Immunohistochemical staining showed that the decreased Cu, Zn-SOD staining pattern was observed in the basal cell layr corresponding to the dermal papillae.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] H.Iizuka,H.Takahashi: "Psoriasis,involucrin and protein kinase C" International Journal of Dermatology. 32. 333-338 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Takahashi,H.Iizuka: "Retinoid-induced beta-adrenergic inhibitory effect associated with increased thymidine incorporation of FRSK cells" Journal of Dermatological Science. 5. 122-129 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Takahashi,H.Iizuka: "Neither cyclosporin A nor FK506 affects adenylate cyclase responses of FRSK cells at concentrations that inhibit thymidine incorporation" Journal of Dermatological Science. 6. 235-239 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 飯塚一: "表皮細胞の増殖制御:カルモジュリンとその展開" 皮膚病診療. 15. 1034-1038 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 飯塚一,高橋英俊: "表皮のβ-アドレナリンアデニル酸シクラーゼ:グルココルテコイドによる制御" 皮膚科の臨床. 35. 1291-1296 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kobayashi T,Iizuka H et al: "Ultrastructural localization of superoxide dismutase in human skin" Acta Dermatovenereologics. 73. 41-45 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iizuka, H., Asano, K., Ito, F., Tamura, T., Takahashi, H., Tsutsui, M: "Desensitization of the epidermal adenylate cyclase system : agonists and phorbol esters desensitize by independent mechanisms." Biochim Biophys Acta. 1093. 95-101 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iizuka, H., Tahahashi, H.: "Glucocorticoid-induced regulation of beta2-adrenergic receptors in keratinocytes." The Biology of the Epidermis (eds.A.Ohkawara, J.McGuire) Elsevier. 97-105 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iizuka, H., Takahashi, H.: "Psoriasis, involucrin, and protein kinase C." Int J Dermatol. 32. 333-338 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iizuka, H., Asaga, H., Koike, K., Iizuka, S.: "Decreased Cu, Zn-superoxide dismutase activity in psoriatic hyperproliferative epidermis." Eur J Dermatol. 3. 56-58 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahashi, H., Iizuka, H.: "Regulation of beta2-adrenergic receptors in keratinocytes : glucocorticoids increase steady-state levels of receptor mRNA in foetal rat keratinizing epidermal cells." Br J Dermatol. 124. 341-347 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahashi, H., Iizuka, H.: "Analysis of the 5^1-upstream promoter resion of human involucrin gene : activation by 12-o-tetradecanoylphorbol-13-acetate." J Invest Derrmatol. 100. 10-15 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kobayashi, T., Matsumoto, M., Iizuka, H., Suzuki, K., Taniguchi, N.: "Superoxide dismutase in psoriasis, squamous cell carcinoma and basal cell epithelioma : an immunohistochemical study." Br J Dermatol 124. 555-559 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kobayashi, T., Saito, N., Takemori, N., Iizuka, S., Suzuki, K., Taniguchi, N., Iizuka, H.: "Ultrastructural localization of superoxide dismutase in human skin." Acta Derm Venereol. 73. 41-45 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hashimoto, Y., Ohkuma, N., Iizuka, H.: "Reduced superoxide dismutase activity in UVB-induced hyperproliferative pig epidermis." Arch Dermatol Res. 283. 317-320 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hashimoto, Y., Tsutsui, M., Iizuka, H.: "Flow cytometric analysis of pig epidermal kerarinocytes : effects of tape stripping." J Dermatol Sci. 4. 193-201 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Iizuka,H.Takahashi: "Psoriasis,involucrin and protein kinase C" International Journal of Dermatology. 32. 333-338 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Takahashi,H.Iizuka: "Retinoid-induced beta-adrenergic inhibitory effect associated with increased thymidine incorporation of FRSK cells" Journal of Dermatological Science. 5. 122-129 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Takahashi,H.Iizuka: "Neither cyclosporin A nor FK506 affects adenylate cyclase responses of FRSK cells at concentrations that inhibit thymidine incorporation." Journal of Dermatological Science. 6. 235-239 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 飯塚 一: "表皮細胞の増殖制御:カルモジュリンとその展開" 皮膚病診療. 15. 1034-1038 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 飯塚一.高橋英俊: "表皮のβ-アドレナリンアデニル酸シクラーゼ:グルココルテコイドによる制御" 皮膚科の臨床. 35. 1291-1296 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kobayashi T,Iizuka H et al: "Ultrastructural localization of superoxide dismutase in human skin" Acta Dermatovenereologice. 73. 41-45 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Takahashi: "Analysis of the 5′-upstream promoter region of human involucrin gene" J Invest Dermatol. 100. 10-15 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Iizuka: "Decreased Cu,Zn-superoxide dismutase activity in psoriatic hyperproliferative epidermis" Eur J Dermatol. 3. 56-58 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Iizuka: "psoriasis involucrin and protein kinase c" Int J Dermatol.

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.hashimoto: "Flow cytometric analysis of pigepidermal keratinocytes:effects of tape stripping" J Dermatol Sci. 4. 193-201 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 飯塚 一: "phospholipase A_Z 活性の上昇からみた乾癬" 皮膚臨床. 33. 1015-1023 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 飯塚 一: "表皮細胞増殖とSOD" J Act Oxyg Free Rad. 3. 313-321 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Iizuka: "The Biology of the Epidermis" Elsevier, 228 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 飯塚 一: "SODの基礎と臨床 皮膚疾患とSOD" メディカル トリビューン, 142 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Takahashi,H.Iizuka: "Regulation of β_2ーadrenergic receptors in Keratinocytes:glucocorticoids increase steadyーstate levels of receptor mRNA in foetal rat keratinizing epidermal cells" British Journal of Dermatology. 124. 341-347 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Iizuka,K.Asano,F.Ito,T.Tamura,H Takahashi,M.Tsutsui: "Desensitization of the epidermal adenylate cyclase system:agonists and phorbol esters desensitize by independent mechanisms" Biochimica et Biophysica Acta. 1093. 95-101 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Hashimoto,N.Ohkuma H.Iizuka: "Reduced superoxide dismutase activity in UVBーinduced hyperproliferative epidermis" Archives of Dermatological Research. 283. 317-320 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 飯塚 一: "表皮細胞増殖制御因子" 医学のあゆみ. 156. 503-505 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 飯塚 一: "乾癬とアラキドン酸カスケ-ド" 日本皮膚科学会雑誌. 101. 1702-1706 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小泉 洋子,大河原 章,松尾 忍,飯塚 一: "乾癬に対するユイコサペンタエン酸内服・外用治療効果" 日本皮膚科学会雑誌. 101. 1713-1716 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Iizuka,H.Takahashi: "Glucocorticoidーinduced regulation of beta_2ーadrenergic receptors in keratinocytes" Elsevier,

    • Related Report
      1991 Annual Research Report
  • [Publications] 飯塚 一: "尋常性乾癬の病態 表皮細胞の解析" 日本皮膚科学会専門医委員会, 17 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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