• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Isolation of novel extracellualr matrix component and its function related with tissue fragility

Research Project

Project/Area Number 03670527
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionOita Medical University

Principal Investigator

SHINKAI Hiroshi  Oita Medical University Associate Professor, 医学部, 助教授 (90030957)

Co-Investigator(Kenkyū-buntansha) KATAGIRI Kazumoto  Oita Medical University Instructor, 医学部, 助手 (00204420)
FUJIWARA Sakuhei  Oita Medical University Assistant Professor, 医学部, 講師 (90181411)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsExtracellular matrix / 45kD protein / Fibrosis / デコリン
Research Abstract

To isolate novel component of extracellular matrix, new-born calf skin was extracted with 6 M urea or 4 M guanidine. The protein was separated from collagen by ion exchange chromatography and SDS-polyacrylamide gel electrophoresis. The proteins corresponded to Mr of 45 kD were cut from the gel and injected to rabbit. The antibodies did not shown cross reactivity against known extracellular matrix components though, reacted to 45 kD protein. Immunohistochemical findings showed that the protein coexisted around collagen fiber and in endothellial cells. The proteins were synthesized by cultured fibroblasts, though the synthesis was much lower compared to collagen. Three polypeptides were obtained from this protein after CNBr treatment. Following amino acid sequences were shown, DINGGAATLPQKLYQT;WLVNDTAVLP GKLYYVGVGFA ; SPDFAAST LAGLDDATKV from CB-peptides. N-terminal sequences from the protein was corresponded to large fragment of CB-peptides. Several oligonucleotides were synthesized from the amino acid sequence. To isolate the gene from cDNA library from fibroblasts and endothellial cells, antibodies and PCR method using synthesized DNA were used. No cross reactive colonies nor complementaly DNA were obtained from these libraries.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Fujiwara S,Shinkai H,Timpl R: "Structure of N‐linhed aligo saccharide chains in the triple‐helical domains of human type VI and moure type VIII collagin" Matrix. 11. 307-311 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Katagiri,K,Honela.T Matsunaga.E.Shinkai.H: "Conparison of small and large praliogtyceens in bouine and nasal cuiti lage" Connectuie tissue. 22. 99-106 (1991)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi