| Project/Area Number |
03670576
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
YAMAMOTO Tetsu Asahikawa Medical College,Assistant Prof., 医学部, 講師 (50125415)
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| Co-Investigator(Kenkyū-buntansha) |
SAWA Masayuki Asahikawa Medical College,Research Associate., 医学部, 助手 (70226059)
KASAI Shinichi Asahikawa Medical College,Associate Prof., 医学部, 助教授 (40091566)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Artificial Livre / Lipophilic membrane / Lipophilic toxin / Liver Microsome / Encapsulated hegatocyte / Phenol / Sulfide / 被包遊離肝細胞 / 脂溶性毒素 / ディメチルサルファイド / グルクロン酸抱合 / ガラクトサミン肝障害 |
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| Research Abstract |
Lipophilic toxins were known as highly potent agents which induce hepatic coma and selective exclusion of these toxins using lipid membrane technique was investigated in this study. Both Polypropylene and Polyethylene hollow-fiber membranes were used after paraffin oil treatment. Phenol and Dimethylsulfide(DMS) were well known lipophilic toxins and permeate through these lipophilic membranes well but these metabolites were hydrophilic and were not able to permeate through the membranes. Liver microsome was used for DMS oxidation reaction and applied to the system for artificial liver. Encapsulated hepatocytes were also applied to the system for glucuronidation reaction of phenol. Both liver microsome and encapsulated hepatocytes well utilized on exclusion of lipophilic toxins through lipophilic membranes. 30 to 50% of those ability on enzymatic detoxification for lipophilic toxins were accererated by treating the animals which are the source of them. But no additives were beneficial on proliferation and functions of encapsulated hepatocyte. Only continuous addition of the solution containing liver microsomes achieved increasing detoxification unit on artificial liver support using lipophilic membrane.
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