| Project/Area Number |
03670590
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Kyusyu University |
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Principal Investigator |
MAEHARA Yoshihiko Kyusyu University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (80165662)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Hideo Kyusyu University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (20240905)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Intracellular free calcium / Chemosensitivity test / Cytotoxicity / 細胞内カルシウム濃度 |
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| Research Abstract |
Alterations in concentrations of intracellular free calcium([Ca^<2+>]i) were examined in mouse sarcoma S-180 cells exposed to cisplatin, mitomycin C and adriamycin, as related to cell viability. The [Ca^<2+>]i was measured using the fluorescent Ca^<2+>indicator, fura-2. During the first 24 hr of incubation of the cells in vitro, in the presence of the drugs, the [Ca^<2+>]i was lower than in the control cells. After 24 hr of incubation, the [Ca^<2+>]i began to increase at a time when the cell viability was reduced to 50% of the controls, and finally exceeded 1 muM with reduction to 10%. The increase in [Ca^<2+>]i was reciprocal to the loss of the cell viability. Changed in Ca^<2+> concentrations in the medium did not alter the results. The findings suggest that in the presence of anticancer drugs there is an accumulation of free Ca^<2+> with a release of this ion from internal stores and that cell death follows disruption in the homeostasis of intracellular Ca^<2+>.
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