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Anastomotic remodeling after multiple organ transplantation

Research Project

Project/Area Number 03670607
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

SHIGEMATSU Hiroshi (1992-1993)  University of Tokyo Surgery, Lecturer, 医学部・附属病院, 助手 (40134556)

加地 利雄 (1991)  東京大学, 医学部・(病)第1外科, 助手 (40185765)

Co-Investigator(Kenkyū-buntansha) ARAMOTO Haruo  University of Tokyo Surgery, Assistant, 医学部・附属病院, 医員
HATAKEYAMA Takuya  University of Tokyo Surgery, Assistant, 医学部・附属病院, 医員
NUNOKAWA Masao  University of Tokyo Surgery, Assistant, 医学部・附属病院, 医員
OHSHIRO Hidemi  University of Tokyo Surgery, Assistant, 医学部・附属病院, 医員
YASUHARA Hiroshi  University of Tokyo Surgery, Lecturer, 医学部・附属病院, 助手 (50251252)
安原 洋  東京大学, 医学部(病), 助手 (40210273)
小林 一博  東京大学, 医学部・(病)第1外科, 助手 (80205450)
重松 宏  東京大学, 医学部・(病)第1外科, 助手 (40134556)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Keywordsmultiple organ transplantation / vascular anastomosis / anastomotie intimal hyperplasia / FK506 / tissue preservation / microcirculation / reperfusion injury / PDGF / ゴアテックス製代用血管 / 仮性内膜肥厚 / FKー506
Research Abstract

Immunologic mechanisms also have been implicated in the development of pseudointimal hyperplasia which is the major cause of occlusion following vascular surgery using artificial prosthetic grafts in the late postoperative stages.
In this study, we implanted cPTFE grafts into abdominal aortae of dogs by end to end proximal anastomosisand by end to side distal anastomosis. The dogs were then devided into three groups, control group A,group B treated with low dose (0.1mg/kg/week) of FK506 and group C with high dose (0.2mg/kg/week). The first dise was given at the seventh day after surgery and subseaquent weekly injection continued for 6 months. At sixth month, the arotaewas harvested, fixed, longitudinary sectioned and the average width of pseudointima was determined. As compared to control group A,FK506 caused significant reduction in psuedointimal thickness at the proximal and the distal toe side anastomosis in the Group B and C.However, the suppression of pseudointima at the distal hee … More l anastomosis was not significant. These data show that an inhibitory effects of FK506 on pseudointimal hyperplasia following vascular anastomosis has the difference in the site of anastomosis and further suggest the involvement of immunological regulation in the intimal hyperplastic response.
Then, in order to study the efficacy of FK506 on suppression of intimal thickness and to determine the immunohistological effects of FK506, we implanted ePTFE grafts into abdominal aortac of New Zealand White rabbits in the same way as that of dogs above-mentioned, and the rabbits were receiving a 1% choresterol diet 2 weeks before and for 8 weeks after operation and ramdomly devided into three groups, control group D,group E treated with placebo ointment and group F treated with 1% FK506 ointment. Eight weeks later, each entire graft was harvested including the adjacent aortae, longitudinally sectioned and the width of anastomotic pseudointima was determined. The change in the serum cholesterol level was similar among these three groups. Whole blood concentration of FK506 faded away by 5 weeks after surgery. As compared to group D and group E,FK506 ointment caused significant reduction in psuedointimal thickness at every anastomotic sites in group F.These data show that local treatment administration of FK506 ointment, as well as systemic administration, inhibits the pseudointimal hyperplasia following vascular anastomosis. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 重松宏: "人工血管の閉塞-この症例で行うべき検査と治療." 外科. 53(1). 8-15 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 重松宏: "臓器移植の現況-血管移植." 診断と治療. 79(3). 572-576 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 安原洋: "Reprfusion injuryにおける微小循環透過性測定の有用性に関する実験的検討." 日本血管外科学会誌. 1(1). 29-33 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 重松 宏: "腹腔・上腸間膜動脈領域の再建." 手術. 46(12). 1927-1935 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Ohsiro: "Intimal thickening of arteries in gastric walls of those with arteriosclerosis obliterans and abdominal aortic aneurysms." Microcirculation Annual 1991. 69-70 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shigematsu: "Comparison of patency rates of EXS graft (Dacron graft) and technogenic (EPTEF graft) for above-knee femoropopliteal bypasss operations." Vascular Surgery. 1(1). 514-516 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shigematsu: "Inferior mesenteric and hypogastric artery reconstruction to prevent colonic ischemia following abdominal aortic aneurysmectomy." Cardiovascular Surgery. 1(1). 13-18 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.ohshiro: "Intimal thickening of arteies in gastric walls of those with arterioselerosis obliterans and abdominal aortic aneurysms." Microcirculation annual. 1991. 67-70 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shigematsu: "Comparison of patency rates of EXS graft (Dacron graft) and Technograft (EPTFE graft) for above-knee femoropopliteal bypasss poerations." Vascular Surgery. 1(1). 514-516 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shigematsu: "Inferior mesenteric and hypogastric artery reconstruction to prevent colonic ischemia following abdominal aortic aneurysmectomy." Cardiovascular Surgery. 1(1). 13-18 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Yasuhara: "An analysis of heterogeneous albumin leakage due to ischemia-reperfusion injury" Japanese Journal of Vascular Surgery. 1(1). 29-33 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shigematsu: "Prosthetic graft occlusion after revascularlization" Geka. 53(1). 8-15 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shigematsu: "The state of transplantation ; vascular replacement" Diagnosis and Treatment. 79(3). 572-576 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shigematsu: "Vascular reconstructtion of celiac and superior mesenteric arteries" Syujyutu. 46(12). 1927-1935 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary

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Published: 1991-04-01   Modified: 2016-04-21  

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