The effects of ketone body infusion on liver regeneration

Research Project

Project/Area Number	03670609
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Digestive surgery
Research Institution	The Nippon Dental University (1992) Niigata University (1991)
Principal Investigator	YOSHIDA Keisuke The Nippon Dental Univ, School of Dent., at Niigata, Department of Surgery, Professor, 新潟歯学部, 教授 (90018680)
Co-Investigator(Kenkyū-buntansha)	TOMIYAMA Takemi Niigata Univ., School of Med., 1st Department of Surgery, Assistant, 医学部, 助手 KAWAI Chihiro The Nippon Dental Univ, School of Dent., at Niigata, Department of Surgery, Assi, 新潟歯学部, 助教授 (50186045) SHIRAI Yoshio Niigata Univ., School of Med., 1st Department of Surgery, Assistant, 医学部, 助手 (50216173) TSUKADA Kazuhiro Niigata Univ., School of Med., 1st Department of Surgery, Assistant, 医学部, 助手 (90171967)
Project Period (FY)	1991 – 1992
Project Status	Completed (Fiscal Year 1992)
Budget Amount *help	¥2,000,000 (Direct Cost: ¥2,000,000) Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords	Massive hepatectomy / Metabolic change / Liver regeneration / Keton body / Monoacetoacetin / Short chain fatty acid / Liver energy charge
Research Abstract	The effects of glucose based TPN and monoacetoacetin-glucose TPN on metabolic changes and liver regeneration after 70% hepatectomy was investigated in rats. Under ketamine anesthesia male Wistar rats were subjected to 70% hepatectomy. They were divided into three groups according to their TPN diet : 5% glucose group, 20% glucose group and 5% glucose-15% monoacetoacetin group. Liver regeneration was analyzed by remnant liver weight to body weight ratio, mitotic index and labeling index for BrdU positive cells. Body weight change and plasma GPT, bilirubin, total protein, urea nitrogen and creatinine were utilized for general assessment. Liver energy charge was measured six hours after surgery. The labeling indices and the mitotic indices of 5% glucose group and monoacetoacetin-glucose group were higher than those of 20% glucose group. The body weight change and remnant liver weight to body weight ratio of 5% glucose group were lower than those of 20% glucose group and monoacetoacetin-glucose group. There was no significant difference in liver energy charge among these three groups at six hours after surgery. The results of this experiment show a possibility of suppression of liver regeneration by high glucose load. The results also show the failure of maintaining body weight and the low liver weight gain with 5% glucose infusion group. Monoacetoacetin-glucose mixture has no adverse effect on liver energy charge at least six hours after surgery. After massive liver resection, monoacetoacetin-glucose mixture administration presents better effects on liver regeneration and body weight gain than glucose based parenteral nutrition.