Analysis of the T-cells for the specific adoptive immunotherapy.

• Project/Area Number: 03670613
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: University of Tokyo
• Principal Investigator: MASAKI Tadahiko (1993) Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 助手 (30238894); 酒井 敬介 (1991-1992) 東京大学, 医学部(病), 助手 (40186987)
• Co-Investigator(Kenkyū-buntansha): MUTO Yasuhiko Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 医員 ; SANO Talesjo Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 助手 (30215884) ; NAGAWA Hirokazu Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 講師 (80228064) ; KOBORI Ouichiro Faculty of Med, University of Tokyo, Associate Professor, 医学部(病), 助教授 (30110696)
• Project Period (FY): 1991 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: Tumor-draining lymphnode / Cytotoxicity / CD-8 / Adoptive immunotherapy / Anti-CD3 antibody / Interleukin-2 / Interferon-gamma / tumor draining / 養子免疫 / 細胞障害性試験 / インターロイキン2 / インターフェロンガンマ / サイトカイン / T細胞 / CTL / 腫瘍免疫 / IL-2 / 抗CD-3抗体 / ILー2 / CDー3

Research Abstract

Tumor-draining lymphnode (DLN) cells, obtained from C57BL/6 mice with fibrosarcoma (MC-1), were activated and expanded with anti-CD3 monoclonal antibody followed by the culture with recombinant interleukin-2 These cells were non-cytolytic in vitro. However, they showed the specific anti-tumor efficacy to the pulmonary micrometastases of MC-1. The supernatant, obtained by co-culturing the activated DLN cells with MC-1, exhibited the specific production of interferon-gamma(IFN-gamma). The therapeutic effect correlated with the specific IFN-gamma production better than the cytolytic activity.

Research Products

• [Publications] 鮫島 伸一 外7名: "Tumor-draining lymphnocytoを用いた養子免疫療法の検討" BIOTHERAPY. 8. 424-425 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] S.Sameshima et al: "IFN-gamma) production of the tumor-draining lymphnode cell" BIOTHERAPY. 9. 708 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] HIROHISA YOSHIZAWA,KEISUKE SAKAI,ALFRED E.CHANG,SUYU SHU: "Actication by AntiーCD3 of TumorーDraining Lymph Node Cells for Specific Adoptive Immunotherapy" Cellular Immunology. 134. 473-479 (1991)