| Project/Area Number |
03670613
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
MASAKI Tadahiko (1993) Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 助手 (30238894)
酒井 敬介 (1991-1992) 東京大学, 医学部(病), 助手 (40186987)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MUTO Yasuhiko Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 医員
SANO Talesjo Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 助手 (30215884)
NAGAWA Hirokazu Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 講師 (80228064)
KOBORI Ouichiro Faculty of Med, University of Tokyo, Associate Professor, 医学部(病), 助教授 (30110696)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Tumor-draining lymphnode / Cytotoxicity / CD-8 / Adoptive immunotherapy / Anti-CD3 antibody / Interleukin-2 / Interferon-gamma / tumor draining / 養子免疫 / 細胞障害性試験 / インターロイキン2 / インターフェロンガンマ / サイトカイン / T細胞 / CTL / 腫瘍免疫 / IL-2 / 抗CD-3抗体 / ILー2 / CDー3 |
|---|
| Research Abstract |
Tumor-draining lymphnode (DLN) cells, obtained from C57BL/6 mice with fibrosarcoma (MC-1), were activated and expanded with anti-CD3 monoclonal antibody followed by the culture with recombinant interleukin-2 These cells were non-cytolytic in vitro. However, they showed the specific anti-tumor efficacy to the pulmonary micrometastases of MC-1. The supernatant, obtained by co-culturing the activated DLN cells with MC-1, exhibited the specific production of interferon-gamma(IFN-gamma). The therapeutic effect correlated with the specific IFN-gamma production better than the cytolytic activity.
|
