Investigation of functional and morphological chang ischemia damaged-liver and effect of xanthine oxidase

• Project/Area Number: 03670621
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: MORIMOTO Taisuke Kyoto University. F, 医学部, 助手 (60135910)
• Project Period (FY): 1991 – 1992
• Project Status: Completed (Fiscal Year 1992)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: liver / warm ischemia / heptic hemodynamics / liver mitochondria / energy metabolism / xanthine oxidase inhibitor / free radical scavenger / 肝エネルギ-代謝 / 肝循環動態 / 細胞保護作用 / 臨床肝切除 / 臓器保存

Research Abstract

After 60 minutes complete warm ischemia of the canine liver induced by the total hepatic vascular exclusion with active venovenous bypass, the microcirculation of the liver was deteriorated, resulting in portal congestion and the deterioration of systemic hemodynamics. Under such a situation, energy metabolism of the liver evaluated by arterial blood ketone body ratio (AKBR) and adenylate energy charge potential (ECP) could not recover to the preoperative levels, resulting in no survival longer than 6 hours. By contrast, if 100 mg/kg body weight xanthine oxidase inhibitor (allopurinol) was administered 10 min prior to the initiation of warm ischemia, AKBR and ECP could restore to the preoperative level, despite no significant changes in the hepatic hemodynamics compared to the group without allopurinol treatment. Lipid peroxide generation was prevented by the treatment and revealed a survival longer than 12 hours, while there was no effect of allopurinol on the morphological changes in the warm ischemic liver. In conclusion, xanthine oxidase inhibitor has a positive effect on hepatic energy metabolism, despite no positive effects on the hepatic hemodynamics and morphological change.

Research Products

• [Publications] 中野 昌彦: "Preserved mitochondrial function by allo purinol despite deteriorated hemodynamics in warm ischemia-damaged canice lever." Reserch in Experimental Medicine. 192. 389-399 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Masahiko Nakano: "Preserved mitochondrial function by allopurinol despite deteriorated hemodynamics in warm ischemia-damaged canine liver." Res Exp Med. 192. 389-399 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 中野 昌彦: "Preserved mitochondrial function by allopurinol despite deteriorated hemodyhamics in warm ischemia-damaged canine liver." Reserch in Experimental Medicine. 192. 389-399 (1992)
• [Publications] Masahiko Nakano et al.: "Influence of blood reteution in the liver during complete warm ischemia on energy metabolism and hemodynamics after reperfusion" Research in Experimental Medicine.
• [Publications] Mitsuhiro Terasaki et al.: "Hemodynamics and hepatic energy metabolism in canin model of acute hepatic venous occlusion with mesocaval Shunt." Surgery.