| Project/Area Number |
03670655
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
IMAIZUMI Munehisa Nagoya University School of Medicine Associated Professor, 医学部, 助教授 (90109322)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Lung cancer / Fibroblast / Growth-promoting factor / Lymph node metastasis / MTT colorimetric assay / Nude mouse / Tyrosin-specific phosphorylation / 制癌剤感受性試験法 / 上皮性成長因子受容体 / 肺癌培養細胞 / ヌ-ドマウス |
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| Research Abstract |
There are complicated interactions between cancer cells and fibroblasts. In this study, the effects of fibroblasts on lung cancer cells were examined. Nine cell lines of human lung cancer cells and 14 cell lines of fibroblasts were used. Lung cancer cells were incubated in conditioned medium (CM) of fibroblasts, and promotion of the growth of lung cancer cells by CM was examined in vitro by MTTcolorimetric assay. CM of fibroblasts promoted the growth of 5 of the 9 cell lines of lung cancer cells, and the responses to EGE were similar. This effect was observed also in fibroblasts derived from lung tissue of cancer-free individuals. CM similarly promoted the growth of human lung cancer cells transplanted to nude mouse. Extensive lymph node metastasis was observed relatively early in 5 of 6 lung cancer patients from whom fibroblasts with growth-promoting effects were obtained. Moreover, tyrosin-specific phosphorylation in lung cancer cells was accelerated by an additional EGF. Acceleration of the phosphorylation was observed in 13 of 25 operated cases of lung cancer. Six of 17 cases were n_0 and seven of 8 cases were n_1 and n_2. From these findings, the presence of fibroblasts with growth-promoting effects in the lung cancer tissue, and tyrosin-specific phoshorylation in lung cancer cells are considered to be related to extensive lymph node metastasis, and the special responses of lung cancer cells to CM of fibroblasts can be a useful index for prognosis of lung cancer patients.
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