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Migration and Morphological Change of Wounded Endothelial Cells, and Effects of Flow

Research Project

Project/Area Number 03670661
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionYokohama City University School of Medicine

Principal Investigator

IMOTO Kiyotaka  Yokohama City University School of Medicine Asistant, 医学部, 講師 (40203335)

Co-Investigator(Kenkyū-buntansha) KONDOH Jiro  Yokohama City University School of Medicine Asocciate Professer, 医学部, 助教授 (00046038)
松本 昭彦  横浜市立大学, 医学部, 教授 (20045975)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsCultured endothelial cells, / Wound model, / Migration, / Morphological change, / Endothelial growth factors, / Biocompatibility, / 血管内皮細胞 / 細胞遊走
Research Abstract

1. Confluent cultured endothelial cells were scraped and the edge were observed by videomicroscope. migration of each cells were 224<plus-minus>153mum/24hours(mean<plus-minus>S.D.) with rndom walk
Endothelial cells were detached partially after 24 hours incubation in zero calcium medium. Distance of migration was 111<plus-minus>79mum/24hours and less than control experiment without statically significanc
3. Imunohistochemical staining of ICAM-1 and VCAM-1 was negative on endothelial cells around the wound edge.
4. Enhacement of wounded endothalial cells reproduction was examined in collagen gel co-culture system with human tissue. Bovine aortic endothelial cells was seeded on collagen gel mixed with human adipose tisuue, striated muscle, omentum, and vein. Human omentum and striated muscle enhanced endothelial cell replication.
5. In vitro system to examine cell compatibility of bioprosthesis was developd with applicatin of endothelial and fibroblast seeding.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (1 results)

All Other

All Publications (1 results)

  • [Publications] 孟真: "生体処理法による細胞親和性の〓について -エポキシ処理とグルタールアルデヒド処理" 人工臓器 印刷予定. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary

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Published: 1991-04-01   Modified: 2016-04-21  

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