• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

GFAP GENE EXPRESSION IN HUMAN GLIOMA CELL

Research Project

Project/Area Number 03670675
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

WASHIYAMA Kazuo  NIIGATA UNIVERSITY.BRAIN RES.INST., ASSOCIATE PROFESSOR, 脳研究所, 助教授 (00183715)

Co-Investigator(Kenkyū-buntansha) KOIKE Tetsuo  NIIGATA UNIVERSITY.MEDICAL HOSPITAL, LECTURER, 医学部附属病院, 講師 (20158893)
KAMEYAMA Shigeki  NIIGATA UNIVERSITY.BRAIN RES.INST., LECTURER, 脳研究所, 講師 (80186014)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsGFAP / Gene expression / Glioma / In situ hybridization / Northern blot / Cell line / gene expression / glioma / in situ hybridization / cell line
Research Abstract

We have analyzed GFAP expression through successive passages of GFAP-positive human glioma cell line by using the immunohistochemistry and Northern blot hybridization. In one cell line (Case 1), the cultured cells showed stable expression of GFAP protein and mRNA from early to later passage levels. In the other cell line (Case 2), GFAP expression was shown to be less stable. At early passage, nearly 100% of the cultured cells displayed intense GFAP immunoreactivity. After passage 30, however, weakly to negatively stained cells increased gradually in number, and by passage 100, almost all cells had become negative for GFAP. A similar change in GFAP immunoreactivity during successive passages was also observed in each of 7 GFAP-positive clones isolated from this cell line at passage 30 and 50, suggesting that each cell had a tendency to reduce and finally stop its GFAP expression. Northern blot studies revealed that GFAP mRNA also change concomitantly in the parental and clonal cell lines, suggesting a regulatory change at the transcriptional level.
GFAP mRNA have been analyzed in materials of one astrocytoma case, and of one anaplastic glioma case using in situ hybridization technique. Anti-sense oligomer specific for GFAP cDNA was selected from 3' non-coding region. The use of this probe with low stringency showed 3.4-kb band in human glioma cell lines by Northern blot hybridization. The positive grains specific for GFAP mRNA were distributed in Bergmann's glia in normal cerebellum, and in astrocytoma tissues, but not in anaplastic glioma tissues.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] K.ONDA: "Extablishment of human glioma cell lines with stable and unstable GFAP expession" Biomedical Research. 13. 327-334 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.KUMANISHI: "Human glial fibrillary acidic protein (GFAP):molecular cloning of the complete cDNA sequence and chromosomal localization (chromosome 17) of the GFAP gene" Acta Neuropathologica. 83. 569-578 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Onda: "Establishment of human glioma cell lines with stable and unstable GFAP expression." Biomedical Research. 13. 327-334 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Kumanishi: "Human glial fibrillary acidic protein (GFAP): molecular cloning of the complete cDNA sequence and chromosomal localization (chromosome 17) of the GFAP gene." Acta Neuropathologica. 83. 569-578 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi