| Project/Area Number |
03670713
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Teikyo University |
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Principal Investigator |
MIKI Hiroshi Teikyo Univ., Medicine, Orthopedics, Assistant Professor, 医学部, 助教授 (70082154)
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| Co-Investigator(Kenkyū-buntansha) |
PARK Pyoyun Teikyo Univ., Medicine, Orthopedics, Assistant, 医学部, 助手 (20147094)
UNNO Kazutoshi Teikyo Univ., Medicine, Orthopedics, Research Associate, 医学部, 教務職員
OHNO Tohgo Teikyo Univ., Medicine, Orthopedics, Professor, 医学部, 教授 (70082120)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Fibrous long-spacing collagen / basal laminae / laminin / type VI collagen / 6型コラーゲン / 免疫電顕 / Fibrous longーspacing collagen / 長周期膠原線維 / VI型コラ-ゲン |
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| Research Abstract |
We are analyzed the distribution of type I , III,IV,V,VI collagens, laminin and fibronectin in fibrous long-spacing collagen (FLS) of collagenase-treated newborn mouse skin, schwnnoma and cultured fibroblasts by immunohistochemistry at the electron microscopic level to clarify the morphogenesis and origin of the fibers.
In the enzyme-treated mouse dermis, we observed the presence of type VI collagen and laminin. The distribution of type VI collagen were recognized in thin filaments with a diameter of 6 nm, unformed material in the vicinity of basal laminae and collagen fibers and the longitudinally aligned filaments of FLS.In staining of laminin, we observed the more positive reaction with the antibody on the basal laminae of capillaries and basal layr cells. The dark bands of FLS, which were electron-dense amorphous materials with a periodicity of 100nm, were stained densely and specifically.
In the schwannoma, we observed the presence of laminin, but not the others. The distribution of laminin were recognized in basal laminae of capillaries, some basal laminae of schwan cell and dark bands of FLS.
In the matrix of culltured fibroblasts, we observed the presence of type VI collagens. This results corresponded with the Brun's study. Studying of the distribution of laminin, there aren't laminin.
These results indicate that the dark bands of FLS may be formed by precipitation of breakdown products from the demal basal laminae partially digested by collagenase at the type VI collagen-contained filaments with the periodic intervals of about 90nm.
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