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Morphogenesis of fibrous long-spacing collagen and tumor metastasis

Research Project

Project/Area Number 03670713
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionTeikyo University

Principal Investigator

MIKI Hiroshi  Teikyo Univ., Medicine, Orthopedics, Assistant Professor, 医学部, 助教授 (70082154)

Co-Investigator(Kenkyū-buntansha) PARK Pyoyun  Teikyo Univ., Medicine, Orthopedics, Assistant, 医学部, 助手 (20147094)
UNNO Kazutoshi  Teikyo Univ., Medicine, Orthopedics, Research Associate, 医学部, 教務職員
OHNO Tohgo  Teikyo Univ., Medicine, Orthopedics, Professor, 医学部, 教授 (70082120)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsFibrous long-spacing collagen / basal laminae / laminin / type VI collagen / 6型コラーゲン / 免疫電顕 / Fibrous longーspacing collagen / 長周期膠原線維 / VI型コラ-ゲン
Research Abstract

We are analyzed the distribution of type I , III,IV,V,VI collagens, laminin and fibronectin in fibrous long-spacing collagen (FLS) of collagenase-treated newborn mouse skin, schwnnoma and cultured fibroblasts by immunohistochemistry at the electron microscopic level to clarify the morphogenesis and origin of the fibers.
In the enzyme-treated mouse dermis, we observed the presence of type VI collagen and laminin. The distribution of type VI collagen were recognized in thin filaments with a diameter of 6 nm, unformed material in the vicinity of basal laminae and collagen fibers and the longitudinally aligned filaments of FLS.In staining of laminin, we observed the more positive reaction with the antibody on the basal laminae of capillaries and basal layr cells. The dark bands of FLS, which were electron-dense amorphous materials with a periodicity of 100nm, were stained densely and specifically.
In the schwannoma, we observed the presence of laminin, but not the others. The distribution of laminin were recognized in basal laminae of capillaries, some basal laminae of schwan cell and dark bands of FLS.
In the matrix of culltured fibroblasts, we observed the presence of type VI collagens. This results corresponded with the Brun's study. Studying of the distribution of laminin, there aren't laminin.
These results indicate that the dark bands of FLS may be formed by precipitation of breakdown products from the demal basal laminae partially digested by collagenase at the type VI collagen-contained filaments with the periodic intervals of about 90nm.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 海野 和俊: "Fibrous long-spacing collagenの免疫電顕" 医学・生物学電子顕微鏡技術研究会誌. 5(2). 13 ((1991))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 三木 浩: "コラゲナーゼ処理したマウスの皮膚におけるFibrous long-spacing collagenの形態形成と起源" 帝京医学雑誌. 15. 291-307 ((1992))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazutoshi UNNO: "Involvement of type VI collagen in fine filaments of fibrous long spacing fibers formed in collagenase-treated mouse skin." J.Clin.Electron Microscopy. 25. 737 ((1992))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 海野 和俊: "Pre-embedding免疫電顕法-特に細胞間質(Fibrous long-spacing collagen)について-" 医学・生物学電子顕微鏡技術研究会誌. 7(2). 68-69 ((1993))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi MIKI: "Morphogenesis and origin of fibrous long-spacing collagen fibers in collagenase-treated mouse skin tissues." Tissue & Cell. 25. 669-680 ((1993))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] UNNO Kazutoshi, MIKI Hiroshi, PARK Pyoyun and OHNO Tohgo: "Immunoelectron microscopy of fibrous long-spacing collagen." J.Technical research conference for medical and biological electron microscopy. 5(2). 13 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] MIKI Hiroshi: "Morphogenesis and origin of fibrous long-spacing collagen fibers in collagenase-treated mouse skin tissues." Teikyo medical journal. 15. 291-307 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] UNNO Kazutoshi, MIKI Hiroshi, PARK Pyoyun and OHNO Tohgo: "Involvement of type VI collagen in fine filaments of fibrous long spacing fibers formed in collagenase-treated mouse skin." J.Clin.Electron Microscopy. 25. 737 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] UNNO Kazutoshi, MIKI Hiroshi, PARK Pyoyun and OHNO Tohgo: "Pre-emmbeding immunoelectron microscopic method. - For extracellular matrix (fibrous long-spacing collagen)-" J.Technical research conference for medical and biological electron microscopy. 7(2). 68-69 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] MIKI Hiroshi, UNNO Kazutoshi, PARK Pyoyun, OHNO Tohgo and NAKAJIMA Mitsuo: "Morphogenesis and origin of fibrous long-spacing collagen fibers in collagenase-treated mouse skin tissues." Tissue & Cell. 25. 669-680 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 海野 和俊: "Fibrous long-spacing collagenの免疫電顕" 医学・生物学電子顕微鏡技術研究会誌. 5(2). 13 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三木 浩: "コラゲナーゼ処理したマウスの皮膚におけるFibrous long-spacing collagenの形態形成と起源" 帝京医学雑誌. 15. 291-307 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kazutoshi UNNO: "Involvement of type VIcollagen in fine filaments of fibrous long spacing fibers formed in collagenase-treaed mouse skin." J.Clin.Electron Microscopy. 25. 737 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野 和俊: "Pre-embedding 免疫電顕法-特に細胞間質(Fibrous long-spacing collagen)について" 医学・生物学電子顕微鏡技術研究会誌. 7(2). 68-69 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi Miki: "Morphogenesis and origin of fibrous long-spacing collagen fibers in collagenase-treated mouse skin tissues." Tissue & Cell. 25. 669-680 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三木 浩: "コラゲナーゼ処理したマウスの皮膚におけるfibrous long-spacing collagenの形態形成と起源" 帝京医学雑誌. 15. 291-307 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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