| Project/Area Number |
03670743
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Akita University |
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Principal Investigator |
HARADA Tadashi Akita Univ. Sch. of Med. Dept. of Urology, Associate Professor, 医学部, 講師 (00108953)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAGATA Shigeru Akita Univ. Sch. of Med. Dept. of Urology, Assistant Professor, 医学部, 助手 (00157596)
NISHIZAWA Osamu Akita Univ. Sch. of Med. Dept. of Urology, Associate Professor, 医学部, 講師 (60091815)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Prostate cancer / Growth factor / Bombesin / GRP / Autocrine growth factor |
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| Research Abstract |
Bombesin(BMBS), a tetradecapeptide extracted from frog skin, was evaluated with its antibody in vitro and in vivo to evaluate growth modulating effects on the DU-145 and PC-3 human prostate carcinoma cell line. The two cell lines were maintained in DME medium containing 2% fetal calf serum and 1 mg/ml insulin in a 37^oC humidified atmosphere of 5% CO_2 and 95% air. BMBS caused a definite effect on a dose-dependent increase in DU-145 and PC-3 cell growth. The addition of antibody to BMBS, called Ab-BMBS rabbit antiserum during the lag phase of growth, resulted in the specific inhibition of DU-145 and PC-3 growth, with or without BMBS.
In in vivo experiments, nude mice were implanted with DU-145 cells in order to evaluate the suppressing effects of Ab-BMBS on carcinoma cells. After the inoculation of 107 DU-145 cells into each mouse, 20mg Ab-BMBS was intraperitoneally injected into the each mouse three times weekly, for three weeks. In mice administered with Ab-BMBS, the growth of DU-145 was markedly suppressed. By immunocytochemical study, BMBS immuno-reactivities were detected on DU-145 and PC-3 cells.
These results suggest that BMBS can function as an autocrine growth factor for human prostate carcinoma cells. Furthermore, as the result of xenografts in mice, we have learned that Ab-BMBS may inhibit the growth of human prostate carcinoma.
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