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IMMUNOHISTOCHEMICAL STUDY IN NORMAL/HYPERPLASTIC PROSTATES AND PROSTATE CARCINOMA-INTERMEDIATE FILAMENT,KERATIN-

Research Project

Project/Area Number 03670764
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKANSAI MEDICAL UNIVERSITY

Principal Investigator

KOMATZ Yosuke  KANSAI MEDICAL UNIVERSITY, 医学部, 教授 (90026873)

Co-Investigator(Kenkyū-buntansha) OKADA Hiyoshi  KANSAI MEDICAL UNIVERSITY, 医学部, 助手 (90247908)
NAKA Yuji  KANSAI MEDICAL UNIVERSITY, 医学部, 助手 (30164114)
TSUBURA Airo  KANSAI MEDICAL UNIVERSITY, 医学部, 教授 (90098137)
MORII Sotokichi  KANSAI MEDICAL UNIVERSITY, 医学部, 教授 (10077563)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsPROSTATE CANCER / LUMINAL CELL / BASAL CELL / KERATIN / ACTIN / Vimentin / Prostate carcinoma / cytokeratin / chromogranin A / Laminin / Prostate cavcinoma / neuro endocvine cells / laminin. / Keratin / Actin
Research Abstract

Immunoreactivities in 25 cases of prostatic adenocarcinoma and 10 normal/hyperplastic prostates were investigated in methacarn-fixed, paraffin-embedded serial sections using a panel of nine anti-keratin monoclonal antibodies (mAbs) ; 34betaE12, CK8.12,312C8-1, CK4.62, RPN1165, RPN1162,35betaH11, CK5, M20, and one of anti-actin mAb, HHF35. In normal/hyperplastic prostates, RPN1162,35betaH11, CK5, M20 stained luminal cells without staining basal cells, and 34betaE12, CK8.12,312C8-1 stained basal cells but not luminal cells. Other mAbs, CK4.62 and RPN1165, stained basal cells as well as luminal cells. All of the mAbs labeling luminal cells stained cancer with variable frequencies in a manner unrelated to the grade of tumor differentiation. Of the prostate cancer cases 92% were scored positive with M20,84% with 35betaH11,80% with CK5,68% with CK4.62,60% with RPN1165 and 4% with RPN1162. However, basal cell-specific keratin labelled with 34betaE12, CK8.12,312C8-1 were totally negative in the cancer cells. HHF35 showed no labelling in normal, hyperplastic or neoplastic epithelial cells of the prostate. Our findings indicate that the major part of the cells of prostatic adenocarcinoma have keratin phenotypes similar to luminal cells but not basal cells, and that no myoepithelial differentiation can be detected in epithelial cell of the prostate. Thus, mAbs for keratins facilitate the indentification of epithelial cell phenotypes in normal, benign and malignant condition of the prostate.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Hiyoshi Okada: "Kevatin profile in nomal/hypcrplcstic proscates and prostate carciuima" Virchows Archiv A Pathol Anat. 421. 157-161 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岡田日佳: "前立腺癌における基底膜の変化と転移" Oncologyの進歩. 2. 16-19 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] HIYOSHI OKADA: "KERATIN PROFILE IN NORMAL/HYPERPLASTIC PROSTATES AND PROSTATE CARCINOMA." VIRCHOWS ARCHIV A PATHOL ANAT. 421. 157-161 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岡田 日佳: "前立腺癌におけるクロモグラニンA陽性細胞の動態" 第80回日本秘尿器科学会総会. 337- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡田 日佳: "前立腺癌における基底膜の変化ーラミニンによる免疫組織学的検討ー" 日本病理学会会誌.

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡田 日佳: "前立腺癌の免疫組織学的研究(I)ケラチンについて" 日本病理学会会誌. 80. 346 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岡田 日佳: "前立腺癌の免疫組織学的研究(II)ケラチンについて" 日本癌学会総会記事. 50. 331 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岡田 日佳: "ヒト前立腺癌組織におけるアクチン及びビメンチンの免疫組織化学的観察(Immunohistochemical demonstration of actin and vimentin in human prostatic carcinoma tissues)" Acta Histochemica et Cytochemica. 24. 531 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiyoshi Okada: "Keratin profiles in normal/hyperplastic prostates and prostate carcinoma" Vivchows Archiv A.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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