| Project/Area Number |
03670773
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KUBOTA Toshiro Tokyo Medical and Dental Univ., Lecturer, 医学部・産婦人科学, 講師 (50126223)
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| Co-Investigator(Kenkyū-buntansha) |
KAMADA Shusaku Tokyo Medical and Dental Univ., Research-Associate, 医学部・産婦人科学, 助手 (80169606)
ASO Takeshi Tokyo Medical and Dental Univ., Professor, 医学部・産婦人科学, 教授 (60093176)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | endothelin-1 / Granulosa cells (purcine) / cell culture / steroid hormone / cell proliferation / cyclic AMP / endothein receptor / intracellular signal transduction / ノーザン解析 / エンドセリンー1 / 顆粒膜細胞 |
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| Research Abstract |
This study was conducted to investigate whether endothelin-1 (ET-1) has direct effects on the functions of porcine granulosa cells harvested from small or medium follicles. The positive ET-I-like immunoreactivity (ET-1-LI) was observed in the cultured porcine granulosa cells, and Northern blot analysis demonstrated the expression of messenger RNA for prepro-ET-1 in these cells. THe binding study showed the presence of non-selective, single class of binding sites which included subtype of ET_B, predominantly. Increased ET-1-LI was detected in the human follicular fluid obtained from the patients in stimulated ovarian cycles. ET-1 stimulated basal and FSH-stimulated secretion of progesterone during short term incubation (2 hrs), while it inhibited FSH- and hCG-stimulated accumulation of progesterone during long-term incubation (48 hrs) of immature or moderately mature granulosa cells. ET-1 significantly increased DNA synthesis in the cells. These biological actions were induced by ET-1, probably via cAMP - protein kinase A pathway and intracellular calcium mobilization - protein kinase C pathway. The results suggest that ET-1 exerts autocrine/paracrine effects on the porcine granulosa cell functions.
Furthermore, the present study also demonstrates that human decidual cells in the early pregnancy can synthsize and release ET-1, and possess specific receptors for ET-1 functionally coupled to phosphoinositide breakdown. This suggests that ET-1 may have its potential autocrine and/or paracrine function in human decidual cells.
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