• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of endothelin-I on the function of the cultured granulosa sells

Research Project

Project/Area Number 03670773
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUBOTA Toshiro  Tokyo Medical and Dental Univ., Lecturer, 医学部・産婦人科学, 講師 (50126223)

Co-Investigator(Kenkyū-buntansha) KAMADA Shusaku  Tokyo Medical and Dental Univ., Research-Associate, 医学部・産婦人科学, 助手 (80169606)
ASO Takeshi  Tokyo Medical and Dental Univ., Professor, 医学部・産婦人科学, 教授 (60093176)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsendothelin-1 / Granulosa cells (purcine) / cell culture / steroid hormone / cell proliferation / cyclic AMP / endothein receptor / intracellular signal transduction / ノーザン解析 / エンドセリンー1 / 顆粒膜細胞
Research Abstract

This study was conducted to investigate whether endothelin-1 (ET-1) has direct effects on the functions of porcine granulosa cells harvested from small or medium follicles. The positive ET-I-like immunoreactivity (ET-1-LI) was observed in the cultured porcine granulosa cells, and Northern blot analysis demonstrated the expression of messenger RNA for prepro-ET-1 in these cells. THe binding study showed the presence of non-selective, single class of binding sites which included subtype of ET_B, predominantly. Increased ET-1-LI was detected in the human follicular fluid obtained from the patients in stimulated ovarian cycles. ET-1 stimulated basal and FSH-stimulated secretion of progesterone during short term incubation (2 hrs), while it inhibited FSH- and hCG-stimulated accumulation of progesterone during long-term incubation (48 hrs) of immature or moderately mature granulosa cells. ET-1 significantly increased DNA synthesis in the cells. These biological actions were induced by ET-1, probably via cAMP - protein kinase A pathway and intracellular calcium mobilization - protein kinase C pathway. The results suggest that ET-1 exerts autocrine/paracrine effects on the porcine granulosa cell functions.
Furthermore, the present study also demonstrates that human decidual cells in the early pregnancy can synthsize and release ET-1, and possess specific receptors for ET-1 functionally coupled to phosphoinositide breakdown. This suggests that ET-1 may have its potential autocrine and/or paracrine function in human decidual cells.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] S.Kamada et al.: "Direct effect of endothelin-I on the granulosa cells of the porcine ovary." Journal of Endocrinology. 134. 59-66 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Kubota et al.: "Synthesis and release of endothelin-I by human decidual cells" Journal of Clinical Endocrinology and Metabolism. 75. 1230-1234 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Kamada et al.: "Endothelin-I is an autocrine/paracrine regulator of porcine granulosa cells" Journal of Endocrinological Investigation. 16. 425-431 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Kamada et al.: "High levels of immunoreactive endothelin-I in human follicular fluids." Human Reproduction. 8. 674-677 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Kubota et al.: "Protein kinase C activation and calcium mobilization decrease prolactin release from human decidual cells" Journal of Endocrinology. 137. 335-340 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 久保田俊郎 他: "エンドセリンと生殖内分泌" ホルモンと臨床. 41. 221-226 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Journal Kamada, S., Kubota, T., Hirata, Y., Taguchi, M., Eguchi, S., Marumo, F.and Aso, T.: "Direct effect of endothelin-I on the granulosa cells of the porcine ovary." Journal of Endocrinology. 134(1). 59-66 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kubota, T., Kamada, S., Hirata, Y., Eguchi, S., Imai, T., Marumo, F.and Aso, T.: "Synthesis and release of endothelin-1 by human decidual cells" Journal of Clinical Endocrinology and Metabolism. 75(5). 1230-I234 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamada, S., Kubota, T., Hirata, Y., Imai, T., Ohta, K., Taguchi, M., Marumo, F.and Aso, T.: "Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells." Journal of Endocrinological Investigation. 16(6). 425-431 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamada, S., Kubota, T., Taguchi, M.and Aso, T.: "High levels of immunoreactive endothelin-1 in human follicular fluids." Human Reproduction. 8(4). 674-677 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kubota, T., Kamada, S., Taguchi, M., Sakamoto, S.and Aso, T.: "Protein kinase C activation and calcium mobilization decrease prolactin release from human decidual cells in early pregnancy" Journal of Endocrinology. 137(2). 335-340 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kubota, T., Kamada, S.and Aso, T.: "Roles of endothelin-1 on the porcine granulosa cells as a local regulator in the ovary." Hormone Research, in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohta, K., Hirata, Y., Shichiri, M., Ichioka, M., Kubota, T., Marumo, F.: "Cisplatin-induced urinary endothelin excretion." JAMA. 265. 1391-I392 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kubota, T., Taguchi, M., Kamada, S.and Aso, T.: Hormonal and immunological factors on local control of implantation in humans. In Perspectives on assisted reproduction : Frontiers in Endocrinology, Vol.4, Ares-Serono Symposia.Christengraf S.r.l., Rome, Italy, (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Kamada et al.: "Direct effect of endothelin-1 on the granulosa cells of the porcine cvary" Journal of Endocrinology. 134. 59-66 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Kubota et al.: "Synthesis and release of endothelin-1 by human decidual cells" Journal of Clinical Endocrinology and Metabolism. 75. 1230-1234 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kamada et al.: "Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells" Journal of Endocrinological Investigation. 16. 425-431 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kamada et al.: "High levels of immunoreactive endothelin-1 in human follicular fluids" Human Reproduction. 8. 674-677 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 久保田俊郎 他: "エンドセリンと生殖内分泌" ホルモンと臨床. 41. 221-226 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Kubota et al.: "Protein Kinase C activation and Calcium mobilization decrease prolactin release from human decidual cells in early pregnancy" Journal of Endocrinology. 137. 335-340 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shusaku Kamada: "Direct effect of endothelin-1 on the granulosa cells of the porcine ovary" Journal of Endocrinology. 134. 59-66 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiro Kubota: "Synthesis and release of endothelin-1 by human decidual cells" Journal of clinical Endocrinology and Metabolism. 75. 1230-1234 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 鎌田 周作: "培養ブタ顆粒膜細胞(PGC)におけるエンドセリン-3の作用についてーET-1との比較ー" 日本不妊学会雑誌. 36. 1087- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 鎌田 周作: "培養ブタ顆粒膜細胞(PGC)におけるエンドセリン-1のプロゲステロン分泌促進作用について。" 日本内分泌学会雑誌. 67. 471- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 久保田 俊郎: "ヒト脱落膜細胞からのEndothelin-1(ET-1)およびET-3の作用発現に関する研究" 日本産科婦人科学会雑誌. 44. S-251- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 鎌田 周作: "ヒト卵胞液中におけるEndothelin-1(ET-1)とその存在意義に関する検討" 第9回日本受精着床学会学術講演会抄録集. 24- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 鎌田 周作: "卵巣顆粒膜細胞に対する血管収縮物質エンドセリンー1の関与について" 日本不妊学会雑誌. 35. 890 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 鎌田 周作: "培養ブタ顆粒膜細胞(PGC)におけるエンドセリンー1(ETー1)のプロゲステロン分泌促進作用について" 日本内分泌学会雑誌. 67. 471 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久保田 俊郎: "ヒト脱落膜細胞からのEndothelinー1(ETー1)分泌に及ぼすProtein kinase Cの影響" 日本内分泌学会雑誌. 67. 980 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 坂本 秀一: "ヒト脱落膜細胞におけるEndothelinー1(ETー1)分泌能の免疫組織学的ならびに分子生物学的検討" 日本産科婦人科学会雑誌. 44. S-250 (1992)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi