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Immunological study of head and neck cancer

Research Project

Project/Area Number 03670820
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionTokai University School of Medicine

Principal Investigator

MIYAKE Hirosato  Tokai Univ., E.N.T., Professor., 医学部・耳鼻咽喉科, 教授 (60055720)

Co-Investigator(Kenkyū-buntansha) KIMURA Hideshige  Tokai Univ., E.N.T., Asistant., 医学部・耳鼻咽喉科, 助手 (80054039)
YAMAUCHI Susumu  J.college of N.M.T., Asso.Prof., 医療技術短期大学, 助教授 (50166576)
Project Period (FY) 1990 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Keywords: Head and neck cancer / Tumor marker / Immunohistochemical detection / IEP / False positive / Anti SCC associated antibody / Commercial antibodies / 頭頚部癌 / 扁平上皮癌関連抗原 / 株化樹立扁平上皮癌細胞 / 等電点分画 / 免疫組織化学的診断 / 癌の血清学的診断法 / ドットブロット法 / 自宅株化樹立上顎扁平上皮細胞 / 早期診断 / 皮疫組織化学的診断 / 単一分画癌関連抗原 / ロトフォア
Research Abstract

A novel squamous cell cancer associated antibody (SCCAAb) was developed, since detection of SCC associated antigens (SCCAA) against the cells and tissues of SCC of head and neck is highly effective for early diagnosis.
SCCAAb was prepared by immunization to rabbit with specific components from pharyngeal SCC cell Line.
Western blot analysis revealed that SCCAA consist of four components from 120KD to 225KD.
Immunological technique by ABC was applied and SCCAAb, commercial anti-bodies ( Involcrin, Keratin, EGF-R, EMA, PKK2, K-ras (P21) ) were used as first antibodies.
Following results were obtained.
Positive number and percent were 34/40 (85%) with SCCAAb, 28/34 (82%) with Involcrine, 29/30 (97%) with Kratin, 26/39 (67%) with EGF-R, 25/37 (68%) with EMA, 26/37 (70%) with PKK2, and 15/37 (1%) with K-ras (P21). False positive number and percent in benign specimens, 11% with SCCAAb, 42% with Involcrine, 85% with Kratin, 34% with EGF-R 28% with EMA, 49% with PKK2 and 18% with K-ras (P21), respectively.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 山内進: "子宮頸部癌の早期細胞診断に関する免疫学的研究" 東海大学短期大学紀要. 27(in press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 三宅浩郷: "頭頸部癌の免疫化学療法" 日本医事新報. 3552. 13-19 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Susumu Yamauchi: "Immunological Study on cytological detection of Uterine cervical cancer of early stage." Proceeding of the Junior college Tokai Univ.Vol.27(in press). (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirosato Miyake: "Immunological therapy of head and neck cancer." Japan Medical Journal.Vol.3552. 13-19 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 山内進: "子宮頚部癌の早期細胞診断に関する免疫学的研究" 東海大学短期大学紀要. 27. in press (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三宅浩郷: "頭頚部癌の免疫化学療法" 日本医事新報. 3552. 13-19 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山内 進: "上顎癌より分離された扁平上皮癌関連抗原" 臨床免疫. 18. 548-554 (1986)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山内 進: "子宮頸癌発生過程における新しい扁平上皮癌関連抗原の発現に関する免疫組織化学的研究" 日産婦誌. 43. 73-79 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 三宅 浩郷: "頭頸部癌の免疫療法" 8. 1-4 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 三宅 浩郷: "上顎癌の治療をめぐる諸問題" 港北出版印刷, 194 (1984)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山内 進: "上顎癌より分離された扁平上皮癌関連抗原" 臨床免疫. 18. 548-554 (1986)

    • Related Report
      1991 Annual Research Report
  • [Publications] 山内 進: "子宮頚癌発生過程における新しい扁平上皮癌関連抗原の発現に関する免疫組織化学的研究" 日産婦誌. 43. 73-79 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 三宅 浩郷: "頭頚部癌の免疫療法" 免疫アネルギ-. 8. 1-4 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 三宅 浩郷: "上顎癌の治療をめぐる諸問題" 港北出版印刷, 194 (1984)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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