Dejtnerution and proliferative membrant of glial cells cue to retinul dtuc*at

• Project/Area Number: 03670828
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: MIYAO Matsumura Kyoto Univ. Faculty of Medicine assistant prof, 医学部, 講師 (30144380)
• Co-Investigator(Kenkyū-buntansha): IWAKI Musayoshi Kyoto Univ. Faculty of Medicine. research fellow, 医学部, 助手 (10093163) ; 岡田 守生 京都大学, 医学部, 助手 (70221140) ; 吉村 長久 京都大学, 医学部, 講師 (70211662)
• Project Period (FY): 1991 – 1992
• Project Status: Completed (Fiscal Year 1992)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000); Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: proliferative diabetic retinopathy / neovascular tissue / immunohistochemical / collagen / GFAP / fibronectin / 増殖性硝子体網膜症 / グリア細胞 / アストロ細胞 / ミューラー細胞 / 増殖糖尿病網腫症 / グリア / 細胞増殖因子

Research Abstract

Intravitreal neovascular tissue in 8 cases of proliferative diabetic retinopathy was investigated using immunohistochemical techniques. All 8 cases yielded positive immunoreactivity for type II collagen (vitreous collagen). The intravitreal neovascular tissue was classified into two groups (A or B), depending upon the distribution of type I collagen. In group A (3 cases), blood vessels were entirely surrounded by vitreous collagen, and in group B (5 cases), the vessels proliferated on one side of a mass of vitreous collagen. Type I and III collagens were distributed diffusely within the extracellular space of the tissue, whereas type IV collagen and fibronectin (FN) formed a basement membrane-like foundation for the newly formed vessels. Glial fibrillary acidic protein (GFAP)-immunoreactive cells were not clearly detected in any of the cases. Neovascular tissue typically proliferated on the posterior vitreous surface (as found in group B), but was also found to penetrate the vitreous gel (as found in group A). As neovascular tissue proliferation proceeded, types I,III and IV collagens and FN were produced. Glial cells (GFAP-positive cells) were not essential for neovascular tissue formation.

Research Products

• [Publications] 細田泰子、岡田守生、松村美代、荻野誠〓、本田孔士、永井: "Intravitreul nevasculur tissue oe prulitcrativc diabctic retivipithy:an iuunia chewbl spdy" Ophthalwic Restarih. 24(5). 260-264 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hosoda Y,Okuda M,Matsumura M,Ogino N,Honda Y and Nagai, T: "International neovuscular tissue of proliferative dinbetic retinoputhy :" 24(5). 260-264 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 細田 泰子: "Intravitreal neovascnlar tissue of proliferative diabetic retinopathy-an immunogisto chemical study" Ophthalmic Research. 24:5. 260-264 (1992)
• [Publications] Yasuko Hosoda: "Immunohistochemical study on intravitreal proliferative tissue of proliferative diabetic retinopathy" Ophthalmic Research. (1992)
• [Publications] Yasuko Hosoda: "Immunohistochemical study on epiretinal membrane of proliferative vitreoretinopathy" Investigative Ophthalmology and Visual Science. 33,Suppl. (1992)
• [Publications] Nagahisa Yoshimura: "PCR studies on the expression on growth factor genes and growth factor receptor genes in culutured human retinal pigment epithelial cells" Investigative Ophthalmology and Visual Science. 33,Suppl. (1992)
• [Publications] Hidenobu Tanihara: "TGFーbeta and binding protein expressed in cultured human retinal pigment epithelial cells" Investigative Ophthalmology and Visual Science. 33,Suppl. (1992)
• [Publications] Munenori Yoshida: "Cultured human retinal pigment epithelial cells produce plateletーderived growth factor and its receptors" 33,Suppl. (1992)