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Development of molecular genetics in oral bacteroides

Research Project

Project/Area Number 03670848
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionKyusyu University

Principal Investigator

NAKAYAMA Koji  Kyusyu University, Faculty of Dentistry Oral Microbiology Associate Professor, 歯学部, 助教授 (80150473)

Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsBacteroides / Porphyromanas / Periodontal disease / Molecular genetics / Superoxide dismutase / 黒色色素産生 / ス-パ-オキシド ジスムタ-ゼ
Research Abstract

(1)Construction of a suicide plasmid (pKDCMZ) for Porphyromonas (Bacteroides) gingivalis.
A suicide plasmid was constructed from a shuttle plasmid vector between E.coli and Bacteroides (pVAL-1). By use of pKDCMZ plasmid, a superoxide dismutase (SOD) -deficient mutant strain was constructed via gene replacement by a mutated sod. The SOD mutant strain showed a rapid viability loss on exposure to air, indicating that SOD is essential for aerotolerance of this organism.
(2)Isolation of colorless mutant strains of P.gingivalis by use of Tn4351 transposon mutagenesis.
Colorless mutant strains of P.gingivalis were isolated by trasconjugation between E.coli harboring R751 : : Tn4351OMEGA4 and P.gingivalis. A gene disrupted by Tn4351 was cloned from one of the colorless mutants and partially sequenced. The amino acid sequence of putative product of the gene (porR) has relatively high similarity with the amino acid sequences of proteins coded by Bacillus stearothermophilus degt, Saccharopolyspora erythraea eryC1, and Streptomyces peucetius dnrJ.These proteins are located at cytoplasmic membrane, functioning as a sensor in signal transduction.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Koji Nakayama: "Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of Porphyromonas gingivalis." Journal of Bacteriology. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koji Nakayama: "A rapid viability loss of an SOD-deficient mutant of Porphyromonas gingivalis on exposure to air." Proceedings of VIth International Conference on Superoxide and Superoxide Dismutase,Elsevier,Amsterdam.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koji Nakayama.: "Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of Porphyromanas gingivalis." J.Bacteriol.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koji Nakayama.: "A rapid viability loss of an SOD-dificient mutant of Porphyromonas gingivalis on exposure to air." Proceedings of VIth International Conference on Superoxide and Superoxide Dismutase, Elsevier, Amsterdam. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koji Nakayama: "Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of porphyromonas gingivalis." Journal of Bacteriology. 176(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中山 浩次: "Porphyromonas gingivalisにおける遺伝子変換の存在" 歯科基礎医学会雑誌. 35. 272- (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中山 浩次: "偏性嫌気性細菌Porphyromonas gingivalisにおける遺伝子変換について" 日本細菌学雑誌. 49. 214- (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中山 浩次: "Porphyromonas gingivalis SOD欠損株の分離とその性状" 日本細菌学雑誌. 48. 151- (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中山 浩次,中山 宏明: "腸内バクテロイデス菌のス-パ-オキシドジスムタ-ゼ(SOD)とPorphyromonas(Bacteroides)gingivalis SODとの類似性" 歯科基礎医学会雑誌. 33. 104 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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