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ヒト唾液腺腺癌細胞が産生する骨形成因子と骨誘導に関する研究

Research Project

Project/Area Number 03670851
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionIwate Medical University

Principal Investigator

HATAKEYAMA Setsuko  Iwate Medical University School of Dentistry Department of Oral Pathology, 歯学部, 助手 (70048495)

Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHeterotopic ossification / Retinic acid / BMP / Cartilage induction / adenocarcinoma cell line / Pleomorophic adenoma / Northern blot / Immunohistochemistry / 免疫組織化学 / ウェスタンブロット / 骨形成因子 / 腺癌株細胞 / 骨形成 / 増殖調節 / オンコジーン / 異所性骨形成 / 軟骨形成 / 無血清培養 / ヌ-ドマウス / 唾液腺腫瘍
Research Abstract

1. Northern and Western blottings demonstrated that human salivary adenocarcinoma cell's subclone (HSG-S8) expressed bone morphogenetic protein (BMP)-2mRNA, and secreted BMP proteins into the medium in serum free culture. BMP-1, -3, and -4 mRAN were not expressed. Retinoic acid (RA, all-trans, 1 mu M) strongly up-regulated the expression of BMP-2 mRNA by the treatment for 6-48 hrs. The BMP-2 proteins in conditined medium also increased 5-fold by the treatment of 1 mu M RA for 48 hrs. Simultaneously, RA suppressed the growth of HSG-S8 cells through down-regulation of the expression of c-myc. Cells did not show particular morphological features of cellular differentiation by treatment of RA.
2. When HSG-S8 cells transplanted into athymic nude mice subcutaneously or intramauscularly, these cells also produced BMP-2 and focally secreted in vivo, which induced heterotopic ossification (s.c.) and cartilage formation (i.m) in the tumos formed by HSG-S8.
3. Immunohistochemistry did not show imunoreations of both of anti-BMP-2(from Genetics Institute R230) and -3(from Genetics Institute, SM10) antibodies to celles of the parotid, submandibular, palatal, and labial glands. Only anti-BMP-1 anti-body immunoreacted to degenerated acinar cells of the submandibular gland. In pleomorphic adenoma, the glandular, solid plasmacytoid, and some spindle tumor cells were positive to anti-BMP(s) antibodies. These findings were observed not only in pleomorphic adenoma with cartilage area, but also without cartilage. In cartilage area, some cells were positive to BMP(s). It was suggested that other factors related to the formation of cartilage area in pleomorphic adenoma.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Setsuko Hatakeyama et al.: "Immunohistochemical localization of bone morphogenetic proteins(BMPs)in salivary gland pleomorphic adenoma" J.Oral Pathol.& Med.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 畠山節子: "上皮性腫瘍による異所性の骨と軟骨の誘導" 岩医大歯誌. 18. 181-188 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Setsuko Hatakeyama: "Expression of bone morphogenetic protein in human adenocarcinoma cell line" Biochem.Biophys.Res.Commun.190. 695-701 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 畠山節子: "唾液腺の多形性腺腫における骨成形因子の免疫組織化学的局在の検索" 歯基礎誌. 35(補). 168 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 畠山節子: "レチノイン酸によるヒト顎下腺由来腺癌細胞株(HSG-S8)のBMP遺伝子発現調節" 日骨代謝誌. 10. 125 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 畠山節子: "レチノイン酸によるヒト顎下腺由来腺癌細胞株(HSG-S8)の増殖抑制とBMPの発現調節" 歯基礎誌. 34(補). 86 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "Immunohistochemical localization of bone morphogenetic proteins (BMPs) in salivary gland pleomorphic adenoma" J.Oral Pathol. & Med.(in press). (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.: "Heterotopic ossification and cartilage formation induced by epithelial neoplasms (Japanese)" Dent.J.Iwate Med.Univ.18. 181-188 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "Expression of bone morphogenetic protein in human adenocarcinoma cell line" Biochem.Biophys.Res.Commun. 190. 695-701 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "Growth suppression and down-regulation of BMP by retionic acid in human adenocarcinoma cell(HSG-S8)(Japanese)" Jpn. j. Biol. 34 suppl. 86 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "Down-regulation of Expression of BMP-2 by retinoic acid in human adenocarcinoma cell(HSG-S8)(Japanese)" JBMM. 10. 125 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al.: "Isolation of salivary adenocarcinoma cell's subclone with in vivo activity of bone and cartilage formation in serum-free cultuer" Jpn. j. Biol. 34. 150-154 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama, S.et al: "Bone formation of subclone in serum-free culture from human salivary gland adenocarcinoma cells(HSG)" Tissue Culture in Dentistry. 26. 13-14 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hatakeyama Setsuko: "Immunohistochemical localization of bone morphogenetic proteins (BMPs) in salivary gland pleomorphic adenoma" J.Oral Pathol.& Med.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 畠山節子: "上皮性腫瘍による異所性の骨と軟骨の誘導" 岩医大歯誌. 18. 181-188 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 畠山節子: "唾液腺の多形性腺腫における骨形成因子の免疫組織化学的局在の検索" 歯基礎誌. 35(補). 168 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hatakeyama Setsuko: "Expression of bone morphogenetic protein in human adenocarcinoma cell line" Biochem.Biophys.Res.Commun.190. 695-701 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Setsuko Hatakeyama et al.: "Expression of bone morphogenetic protein in human adenocarcinoma cell line" Biochem.Biophys.Res.Commun.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 畠山 節子 他: "レチノイン酸によるヒト顎下腺由来腺癌細胞株(HSG-S8)のBMP遺伝子発現調節" 日本骨代謝学会誌. 10. 125- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 畠山 節子他: "レチノイン酸による顎下腺由来腺癌細胞株(HSG-S8)の増殖抑制とBMPの発現調節" 歯科基礎医学会雑誌. 34(補). 86- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 相川 友直他: "ヒト唾液腺癌細胞の培養上清によるラット胎児筋肉細胞の軟骨細胞への誘導" 日本癌学会総会記事 第50回総会. 202- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Setsuko Hatakeyama,et al.: "Bone formation of subclone in serumーfree culture from human salivary gland adenocarcinoma cells (HSG) in athymic nude mouse tumors" Tissue Culture in dentistry. 26. 13-14 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Setsuko Hatakeyama and Atsumi Suzuki: "Isolation of salivary adenocarecinoma cell's subclone with in vivo activity of bone and cartilage formation in serumーfree culture" Jpn.J.Biol.,. 34. 150-154 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Setsuko Hatakeyama et al.: "Bone morphogenetic protein on subcloned human salivary adenocarcinoma cell line in serumーfree culture" Cancer Res.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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