| Project/Area Number |
03670866
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
MAEDA Sadaaki OSAKA UNIVERSITY FACULTY OF DENTISTRY, PHARMACOLOGY. RESEARCH ASSISTANT, 歯学部, 助手 (00135732)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAITO Kihachi OSAKA UNIVERSITY FACULTY OF DENTISTRY, PHARMACOLOGY. ASSOCIATE PROFESSOR, 歯学部, 助教授 (40110788)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Project Status |
Completed (Fiscal Year 1992)
|
| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | Trigeminal ganglion / Opiate receptor / Calcium channel / Enkephalin / Pain transmission |
|---|
| Research Abstract |
Our previous report showed that leucine-enkephalin immunoreactive nerve fibers and cell bodies occurred in trigeminal ganglion of the guinea pig, suggesting the possibility that leucine-enkephalin can modulate pain transmission in trigeminal ganglion. In this research, we characterized opioid receptors and voltage-dependent calcium channels in trigeminal ganglion of giunea pig.
1) The values of Kd and Bmax of ^3H-DPDPE (selective ligand for delta - type opiate receptors) and ^3H-DAMGO (selective ligand for mu - type opiate receptors) were 4.4nM, 29.1 fmol/mg protein and 0.7 nM, 7.2 fmol/mg protein, respectively.
2) The values of Kd and Bmax of ^3H-PN200-110 (L-type calcium channel antagonist) and ^<125>I-omega- conotoxin (N-type calcium channel antagonist) were 48.2 pM, 18.5 fmol/mg protein and 50.1 pM, 29.5 fmol/mg protein, respectively.
3) Acute administration of morphine did not affect both ^3H-PN200-110 and ^<125>I-omega-conotoxin binding. The chronic administration increased the binding of ^<125>I-omega-conotoxin.
4) Using laser scanning fluorescence microscope, we found that leucine-enkephalin-immunorective fibers surrounded some nonimmunoreactive ganglionic cells.
These results suggest that leucine-enkephalin may modulate the function of the primary afferent neuron through calcium dynamics in guinea pig trigeminal ganglion.
|
