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Effects of conditioning stimulation of amygdaloid nucleus on tooth pulp driven-neurons in first somatosensory cortex (SI) of the cat

Research Project

Project/Area Number 03670871
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionIwate Medical University

Principal Investigator

KAWARADA Kei (1993)  Iwate Medical University School of Dentistory Research Associate, 歯学部, 助手 (90204792)

松本 範雄 (1991-1992)  岩手医科大学, 歯学部, 助手 (90048562)

Co-Investigator(Kenkyū-buntansha) 川原田 啓  岩手医科大学, 歯学部, 助手 (90204792)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsLimbic system / Periamygdaloid cortex / Amygdaloid complex / Condisioning Stimulation / Tooth pulp driven neuron / Somatosensory cortex / Nociception / Analgesia / 大脳辺縁形 / 歯髄刺激 / c-fos / 細胞性癌遺伝子 / 視索上核 / 外側手綱核 / 扁桃体中心核 / 開口反射 / グルタメイト
Research Abstract

(1) To demonstrate that the amygdaloid complex modulate the nociception, we observed the effect of amygdaloid conditioning stimulation on the jaw-opening reflex(JOR) regarded as a nociceptive reflex in the cat. The stimulation of the central nucleus(ACE) inhibited the JOR induced by the electrical stimulation of tooth pulp, but the other amygdaloid nuclei did not influence on the reflex. Additionally, further experiments suggested that the JOR inhibitory effect on ACE acts on the trigeminal motor nucleus rather than the sensory nuclei.
(2) We investigated the cat brain sites that were excited by the electrical stimulation of tooth pulp using c-fos immunoreactivity. The number of Fos-positive cells in the tooth pulp-stimulated group increased in the ipsilateral trigeminal caudal nucleus and parabrachial nucleus, and the bilateral lateral habenular nucleus and supraoptic nucleus compared with the anesthetic- or saline- injected groups. The increaes were inhibited by morphine pretreatment.
(3) To elucidate the amygdaloid complex and the surrounding areas have any role in nociception, we studied the effect of conditioning stimulation of these areas on the tooth pulp-driven (TPD) neurons in the first somatosensory cortex of the cat. The stimulation of the amygdaloid nuclei did not alter the activities of TPD neurons as indicated in the result 1. On the other hand, the conditioning stimulation of the periamygdaloid cortex(PAM) located in the ventral side of amygdaloid complex markedly suppressed the slow responses of TPD neurons. Furthermore, this inhibitory effect was antagonized by naloxone.
Results 1 and 3 suggest that the amygdaloid complex and the surrounding area(PAM) decrease the reactions to noxious stimulation by means of the inhibition of both sensory and motor systems.
(4) To investigate the inhibitory sites observed in result 3, we are planing the further studies by the c-fos expression as indicated in result 2.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] K.Kowada,K.Kawarada,N.Matsumoto & T.A.Suzuki: "Inhibitory effect of the central amygdaloid nucleus on the jaw-opening reflex induced by tooth pulp stimulation in the cat." In“Processing and Inhibition of Nociceptive Information"eds.by R.Inoki,Y.Shigenaga and M.Tohyama,Excerpta Medica,Amsterdam. 219-222 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Kowada,K.Kawarada & N.Matsumoto: "Conditioning stimulation of the central amygdaloid nucleus inhibits the jaw-opening reflex in the cat." Jpn.j.Physiol.42. 443-458 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Matsumoto,K.Kawarada,K.Kamata & T.A.Suzuki: "Electrical stimulation of tooth pulp increases the expression of the c-fos in the cat supraoptic nucleus but not in the paraventricular nucleus." Life Science.53. 1235-1241 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Kowada, K.Kawarada, N.Matsumoto and T.A.Suzuki: "Inhibitory effect of the central amygdaloid nucleus on the jaw-opening reflex induced by tooth pulp stimulation in the cat" In "Processing and Inhibition of Nociceptive Information" eds. by R.Inoki, Y.Shigenaga and M.Tohyama, Excerpta Medica, Amsterdam. 219-222 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Kowada, K.Kawarada, and N.Matsumoto: "Coditioning stimulation of the central amygdaloid nucleus inhibits the jaw-opening reflex in the cat" Jpn.J.Physiol. 42(3). 443-458 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Matsumoto K.Kawarada, K.Kamata and T.A.Suzuki: "Electrical stimulation of tooth pulp increases the expression of c-fos in the cat supraoptic nucleus but not in the paraventricular nucleus" Life Sci.53. 1235-1241 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Matsumoto,K.Kawarada,K.Kamata&T.A.Suzuki: "Electrical stimulation of tooth pulp increases the expression of the c-fos in the cat supraoptic nucleus but not in the paraventricular nucleus." Life Science. 53. 1235-1241 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kowada,K.,Kawarada,K.,Matsumoto,N.and Suzuki,T.A.: "Inhibitory effect of the central amygdaloid nucleus on the jaw-opening reflex induced by tooth pulp stimulation in the cat" Processing and Inhibition of Nociceptive Information,Eds.by R.Inoki,Y.Shigenaga and Tohyama,M.,Excerpta Medica,Amsterdam. 219-222 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kowada,K.,Kawarada,K.,Matsumoto,N.and Suzuki,T.A.: "Conditioning stimulation of the central amygdaloid nucleus inhibits the jaw-opening reflex in the cat." Jpan.J.Physiol.42. 443-458 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsumoto,N.,Kawarada,K.,Kamata,K.and Suzuki,T.A.: "Induction of the c-fos proto-oncogene by electrical stimulation of tooth pulp in the cat forebrain." Neurosci.Res.Suppl.17. S255- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松本 範雄,川原田 啓,鎌田 健一,鈴木 隆: "歯髄刺激による間脳内のFos発現部位" 文部省科学研究費総合研究(A)「食物の摂取機構の総合的研究」の班会議抄録集. 4- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松本 範雄,川原田 啓,鎌田 健一,鈴木 隆: "歯髄の電気刺激による前脳内のFos発現部位" Pain Res.7(3). 200- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 古和田 一成,川原田 啓,松本 範雄,鈴木 隆: "開口反射に対する扁桃体条件刺激の効果" 歯基礎誌. 33抄録集. 192 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Kowada,K.Kawahara,N.Matsumoto & T.A.Suzuki: "Inhibitory effect of central amygdaloid nucleus on the jawーopening reflex induced by tooth pulp stimulation in the cat." Abstract of International Symposium of Osaka University for the Celebration of the 50th Aniversay “Processing and Inhibition of Nociceptive Information". 52 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Kowada,K.Kawahara,N.Matsumoto & T.A.Suzuki: "Inhibitory effect of central amygdaloid nucleus on the jawーopening reflex induced by tooth pulp stimulation in the cat." Proceeding of International Symposium on Processing Nociceptive Information.

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Kowada,K.Kawarada,& N.Matsumoto: "Conditioning stimulation of the central amygdaloid nucleus inhibits the jawーopening reflex in the cat." Jpn.J.Physiol.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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