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Molecular-Pharmacological Analysis of Periodontal Disease : Interaction between LPS and Kinins on Microcirculation of Oral Region

Research Project

Project/Area Number 03670879
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

TODOKI Kazuo  Kanagawa Dental College, Pharmacology, Assistant Professor, 歯学部, 講師 (90139577)

Co-Investigator(Kenkyū-buntansha) OKABE Eiichiro  Kanagawa Dental College, Pharmacology, Associate Professor, 歯学部, 助教授 (50097276)
TOMIKAWA Shigeji  Kanagawa Dental College, Instructor (40197918)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsPeriodontal disease / Endotoxin / bradykinin / Lingual artery / Endothelium / nitric oxide / Oxygen free radicals / Prostanoide / 血管平滑筋 / 内皮由来弛緩因子(EDRF) / 電子スピン共鳴法 / エンドトキシン / 内皮細胞由来弛緩因子 / 収縮 / B_<1->受容体 / B_<2->受容体
Research Abstract

In this research project, we wished to determine 1) the effect of bradykinin on lingual artery ; 2) the interaction between LPS and bradykinin on lingual artery and 3) the effect of LPS on transmural nerve stimulation-induced contraction of the isolated mesenteric artery. Oxygen free radical participation in the effect of LPS was investigated. The following results were obtained : 1. Bradykinin-induced contraction may be mediated by an increase of intracellular free Ca ion by B_2 receptors-coupled phosphoinositide (PI) turnover in canine vascular smooth muscle, and bradykinin-induced relaxation may be mediated by a liberated vasodilator substance (e.g.nitric oxide) by the stimulation of the endothelial B_2 receptors, which possibly activates guanylate cyclase to accumulate cGMP in smooth muscle. 2. LPS induces the formation of B_1-kinin receptor in rabbits. The activation of B_1-kinin receptors by des-Arg^9-bradykinin produces contraction of the lingual arteries isolated from the rabbi … More t intravenously treated with LPS.3.LPS inhibits the release of norepinephrine from sympathetic nerve endings by stimulating both prejunctional inhibitory muscarinic- and alpha_2-adrenoceptors. The effect of LPS may involved protein synthesis. 4. LPS by itself can generate oxygen free radicals ( OH radical) via superoxide-dependent and/or Fenton and Harber-Weiss reactions. 5. OH radical selectively damage endothelium-dependent relaxation. It is also postulated that lipid peroxidation may be responsible for this effect.
Finally, it is likely that a sequential activation or/and a modulatory activity of bradykinin, kinin analogous, prostaglandins, and oxygen free radicals via the LPS-interaction is involved in the progression of periodontal disease. Furthermore, one must now begin to put the functional receptor de novo formation hypothesis of the effect of LPS on vascular reactivity in perspective. A through understanding of the endogenous regulatory mechanisms of hemodynamics in oral tissues may be of interest for the development of new concepts of treatment in dental therapeutics in the future. Less

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 杉原 昌実: "Endotoxinによって生ずるB_1-受容体を介した摘出ウサギ舌動脈の収縮反応." 日薬理誌. 98(2). 63-71 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Rusei Taga: "Hydroxyl radical participation in the in vitro effects of Gram-negative endotoxin on cardiac sarcolemmal Na,K-ATPase activity" Japan.J.Pharmacol.55(3). 339-349 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shun-suke Takahashi: "Evidence for generation of hydroxyl radical from Gram-negative endotoxin itself." Dentisry in Japan. 28. 49-53 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shijezi Tomikawa: "Endotoxin impairs the responce of rabbit mesenteric artery to electrical stimulation via a prejunctional mechanism." Br.J.Pharmacol.107(3). 826-832 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Oxygen free radical-mediated selective endothelial dysfunction in isolated coronary artery." Am.J.Physiol.262(2). H806-H812 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Mechanism of bradykinin action in canine lingual arteries."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Proceeding,Oxygen Radicals" Elsevier Science Publishers B.V., (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masami Sugihara: "The contractile response of isolated lingual arteries from rabbits treated with bacterial lipopolysaccharide via the stimulation of B_1-receptors for kinins" Folia pharmacol. Japon.98(2). 63-71 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Rusei Taga: "Hydroxyl radical participation in the in vitro effects of Gram-negative endotoxin on cardiac sarcolemmal Na, K-ATPase activity" Japan.J.Pharmacol.55(3). 339-349 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shun-suke Takahashi: "Evidence for generation of hydroxyl radical from Gram-negative endotoxin itself" Dentisry in Japan. 28. 49-53 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shijeji Tomikawa: "Endotoxin impairs the response of rabbit mesenteric artery to electrical stimulation via a prejunctional mechanism" Br.J.Pharmacol. 107(3). 826-832 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Contraction induced by oxygen free radicals. of coronary artery" 5th International congress on oxygen radicals in Kyoto Japan, Proceeding, 1992 Elsevier Science Publishers B.V. Oxygen Radicals. 449-452

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Oxygen free radical-mediated selective endothelial dysfunction in isolated coronary artery" Am.J.Physiol. 262(2). H806-H812 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Mechanism of bradykinin action in canine lingual arteries" (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Todoki: "Oxygen free radical-mediated selective endothelial dysfunction in isolated coronary artery." Am.J.Physiol.262(2). H806-H812 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 杉原 昌実: "Endotoxinによって生ずるB1-受容体を介した摘出ウサギ舌動脈の収縮反応." 日薬理誌. 98(2). 63-71 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shgeji Tomikawa: "Endotoxin impairs the responce of rabbit mesenteric artery to electrical stimulation via a prejunctional mechanism." Br.J.Pharmacol.107(3). 826-832 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masami Sugihara: "De novo formation of B_<1->kinin receptors in the rabbit induced by E.coli endotoxin" Microcirc.ann.23-24 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sigeji Tomikawa: "The inhibition of electrical stimulationーinduced vasocontraction by E.coli endotoxin" Microcirc.ann.25-26 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 杉原 昌実: "Endotoxinによって生ずるB_<1->受容体を介した摘出ウサギ舌動脈の収縮反応" 日薬理誌. 98. 63-71 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岡部 栄逸朗: "フリ-ラジカルと冠血管の収縮ー弛緩反応" J.Act.Oxyg.Free Rad.2. 690-701 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuo Todoki: "Oxygen free radicalーmediated selective endothelial dysfunction in isolated coronary artery" Am.J.Physiol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuo Todok: "Active Oxygen,Lipid Peroxides and Antioxidants" Elsevier,Amsterdam.The Netherlands., (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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