Evaluation of trial denture cleansers with berberine hydrochloride

• Project/Area Number: 03670916
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 補綴理工系歯学
• Research Institution: HIROSHIMA UNIVERSITY
• Principal Investigator: NAKAMOTO Kyoumi Hiroshima Univ.,Sch.Dent.,Instructor, 歯学部, 助手 (80198259)
• Co-Investigator(Kenkyū-buntansha): TAMAMOTO Mitsuhiro Hiroshima Univ.,Dent.Hospital,Assistant Prof., 歯学部附属病院, 講師 (00136110) ; 二川 浩樹 広島大学, 歯学部, 助手 (10228140)
• Project Period (FY): 1991 – 1992
• Project Status: Completed (Fiscal Year 1992)
• Budget Amount: ¥1,900,000 (Direct Cost: ¥1,900,000); Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: Denture stomatitis / Candida species / Berberine hydrochloride / Denture cleanser / 塩化ベルベリン

Research Abstract

Candida species can be found in high frequency in denture plaque related to denture stomatitis. It is important to clean dentures to prevent denture stomatitis.
This study investigated the antifungal activity of trial denture cleansers against C. albicans, C. tropicalis and C. glabrata. Trial denture cleansers contain berberine hydrochloride. Berberine hydrochloride is a component of crude drugs which have strong antifungal activity and has antifungal activity itself. According to the broth dilution method, the fungicidal activity of cleansers was tested.
One cleanser (S16) that showed activity against C. albicans strongly was tested for Candida removing from acrylic resin plates, color changing of dental materials and surface roughness of acrylic resin plates. These tests revealed that S16 Candida removing activity as well as one commercial denture cleanser and no effects on physical properties.
In vitro studies proved that a trial denture cleanser had antifungal activity and ability to remove adhered fungal cells from acrylic resin surfaces. Hence, use of the cleanser seems to be avail in preventing denture stomatitis.