| Project/Area Number |
03670935
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
外科・放射線系歯学
|
|---|
| Research Institution | TOHOKU UNIVERSITY |
|---|
Principal Investigator |
ECHIGO Seishi Tohoku University, Department of Oral Surgery, Associate Prof., 歯学部口腔外科学第二講座, 助教授 (70005114)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MORI Shiro Tohoku University, Dental Hospital, Assistant Prof., 歯学部附属病院, 助手 (80230069)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Project Status |
Completed (Fiscal Year 1992)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | immune disorder / cytokine / bone morphogenetic protein / bone metabolism |
|---|
| Research Abstract |
Bone formation is under the controls of cytokines in addition to several growth factors including bone morphogenetic proteins (BMPs), suggesting to be regulated by host immune response. To test whether immune disorders in the host influence the bone formation, we analyzed the BMPs-induced bone formation in several strains of mice bearing a single mutation gene; lymphoproliferation gene (lpr) or generalized lymphoproliferative disease gene (gld), which encodes lymphadenopathy, resulting in immune disorders. Crude BMPs derived from bovine bone were injected into femur muscle of these mice, and then quantitative analysis of the ectopic bone formation by using X-ray photography was performed. The expression of the gld gene and to lesser extent the lpr gene had striking potential to enhance the ectopic bone formation in a non-lupus-prone strain of mice, C3H/HeJ. However, in a lupus-prone strain of mice, MRL/MpJ, the lpr gene rather inhibited the ectopic bone formation, possibly due to the enhanced accumulation of osteoclasts histologically observed. These findings indicate that the BMPs-induced bone formation is influenced by immune disorders relevant to lymphoproliferation genes and genetic backgrounds.
|
