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Significance of dipeptidyl peptidases as marker enzyme of oral cancer

Research Project

Project/Area Number 03670944
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionHyogo College of Medicine (1992)
Osaka University (1991)

Principal Investigator

URADE Masahiro  Hyogo College of Medicine, School of Medicine, Associate Professor, 医学部, 助教授 (70104883)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsSerum dipeptidyl peptidase IV / Serum dipeptidyl peptidase II / Tumor-burden marker / Hamster buccal pouch / A model of oral cancer / ハムスター頬襄 / シリアンハムスタ- / ジメチルベンツアントラセン / 頬嚢発癌 / 血清ジペプチジルペプチダ-ゼ活性 / 担癌マ-カ-
Research Abstract

Dipeptidyl peptidases(DPP) are classified into four types, I-IV, according to the substrate specificity. Among them, DPP IV specifically hydrolyzes N-terminal glycylprolyl and is present in microsomal membranes, and DPP II specifically cleaves N-terminal lysylalanyl and is mainly in lysosomes. We have reported that serum DPP IV activity was significantly decreased in oral cancer patients whereas serum DPP II activity was significantly increased, suggesting that they become a possible marker of oral cancer. However, what is the precise mechanism for changing serum enzyme activities and at which stage of carcinogenesis the serum enzyme level begins to be changed, are still unknown.
In the present study, the enzyme expression in the precancerous and cancerous tissues and the change of serum enzyme activity level were investigated using hamster buccal pouch carcinogenesis with 9,10-dimethyl-1,2-benzanthracene (DMBA). The serum DPP IV level was decreased gradually from the 8th to 10th week when papillomas were induced by DMBA application. The enzyme level was further decreased as carcinoma in situ or early invasive carcinoma developed (p<0.001), and reached to less than half of the normal level at the time when tumors were diagnosed as squamous cell carcinoma histologically. This enzyme level was increased by tumor excision and decreased again by tumor recurrence toward death. In contrast, serum DPP II activity showed a minor increase in the early stage of carcinogenesis, and increased significantly as squamous cell carcinoma developed (p<0.01). This enzyme activity tended to change reciprocally to DPP IV activity. Immunohistological staining with rabbit anti-rat DPP IV serum did not detect the significant difference between normal and precancerous or cancerous tissues.
These findings suggest that serum DPP IV and II activities might be useful as tumor-burden markers ; especially, DPP IV activity, which changed from the early stage of carcinogenesis.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] M.URADE: "Serum dipeptidyl peptidase (DPP) IV activity in hamster buccal pouch carcinogenesis with 9,10-dimethyl-1,2-benzanthracene." J.Oral Pathol.Med.21. 109-112 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Urade,M. et al: "Serum dipeptidyl peptidase (DPP) IV activity in hamster buccal pouch carcinogenesis with 9,10-dimethyl-1,2-benzanthracene" J.Oral Pathol. Med. 21. 109-112 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Urade: "Serum dipeptidyl peptidase (DPP) IV activity hamster buccal pouch carcinogenesis with 9,10-dimethyl-1,2-benzanthracene" J. Oral Pathol. Med.21. 109-112 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.URADE et al.: "Serum dipeptidyl peptidase(DPP)IV activity in hamster buccal pouch carcinogenesis with 9,10ーdimethylー1,2ーbenzanthracene" J.Oral Path Med. 21. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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