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Studies on the mechanisms of C9 activation and of C9 inactivation by the regulatory protein(MACIF)

Research Project

Project/Area Number 03671118
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionOsaka Medical College

Principal Investigator

HATANAKA Michiyo  Osaka Medical College, Clinical Pathol., Assistant Prof, 医学部, 助手 (50218484)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Akira  Osaka Medical College, Clinical Pathol., Prof., 医学部, 教授 (00028581)
稲井 真弥  大阪医科大学, 医学部, 教授 (90131317)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsComplement / C9 / Monoclonal antibodies / DTT / GPI-anchor / PNH / DAF / MACIF(CD59) / モノクロ-ナル抗体 / 合成ペプチド / DTT / ジスルフィド結合
Research Abstract

The complement system consist of more than 20 serum proteins and play an important role in host defense. Complement component C9 exerts at the final step of complement cascade by inserting into membranes which contain preformed C9 acceptor, C5b-8. C9 proceeds drastic conformational changes upon interaction with C5b-8 complex. Although the entire primary structure has already been demonstrated, the structual and function relationship is not fully ubderstood. In this research, we intended to clarify the mechanisms of C9 activation by using monoclonal antibodies to C9 and also with chemical modification reagent, DTT. It has been demonstrated that
1. Two seperate epoitipes of C9 are involved in C5b-8 binding.
2. Functional domains of C9 are supported by disulfide bridges.
3. Different and distinct domains of disulfide bridges are implicated in binding to C5b-8, hemolytic activity, and self-polymerization of C9.
Complement functions are targeted only on non-self membranes, and activation does not occur on self membranes. Complement regulating proteins (DAF, MACIF, MCP, CR1) have now been demonstrated to play the crucial roles in self- and non-self determination. We have assesed the function of DAF and MACIF by inccorporating the isolated proteins to PNH erythrocytes which deffect these proteins.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 畑中 道代: "Analysis of C5b-8 binding sites in the C9 molecules using monoclonal antibodies:participation of two seperate epitopes of C9 in C5b-8 binding" Molecular Immunology. 29. 911-916 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 宮川 周士: "Test for ability of decay-accelerating factor(DAF,CD55)and CD59 to alleviate complement-mediated damage of xeno-erythrocytes" Scandinavian Journal of Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 畑中 道代: "Implication of membrane factors other than DAF and CD59 in complement-mediated lysis of paroxysmal nocturnal hemoglobinuria(PNH)erythrocytes" Clinical Immunology and Immunopathology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Michiyo Hatanaka: "Analysis of C5b-8 bindig sites in C9 molecules using monoclonal antibodies : participation of two seperate epitopes of C9 in C5b-8 binding" Molecular Immunology. 29. 911-916 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shuji Miyagawa: "Test for ability of decaryaccelerating factor (CD55) and CD59 to alleviate complement-mediated damage of xenoerythrocytes" Scandinavian Journal of Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Michiyo Hatanaka: "Implication of membrane factors other than DAF and CD59 in complementmediated lysis of paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes" Clinical Immunology and Immunopathology, applied.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 畑中 道代: "Analysis of C56-8 binding sites in the C9 molecules using monoclonal antibodies:Participation of two seperate epitopes of C9 in C56-8 binding" Molecular Immunology. 29. 911-916 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 宮川 周士: "Test for ability of decay-accelerating factor(DAF,CD55) and CD59 to alleviate complement-mediated damage of xeno-erythrocytes" Scandinavian Journal of Immunology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 畑中 道代: "Structural bases for C9 functions,binding to EAClー8 and selfーpolymerization are independent" Complement and inflamation. 8. 157 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 畑中 道代: "Analysis of C5bー8 binding sites in C9 molecules using monoclonal antibodies:Participation of two separate epitopes of C9 in C5bー8 binding" Molecular lmmounol.(1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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