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Molecular analysis of glucose transporter in glomerular mesangial cells

Research Project

Project/Area Number 03671143
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionShiga University of Medical Science

Principal Investigator

KIKKAWA Ryuichi  Shiga University of Medical Science, Third Department of Medicine, Associate professor., 医学部, 助教授 (50093406)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
KeywordsDiabetic nephropathy / Mesangial cells / Glucose transporter / IGF-I / Immunoblotting / Northern blot analysis / Immunoblotting
Research Abstract

It has been known that the abnormalities of mesangial area in glomeruli play a crucial role in the progression of diabetic nephropathy, and the mesangial cells might participate in the evolution of these abnormalities. In order to clarify the role of mesangial cells, the cells were investigated under high concentration of glucose in vitro and various metabolic derangements have been detected, which might be related to the mesangial abnormalities seen in vivo. The glucose appears to cause various.metabolic abnormalities in mesangial cells after transported intracellularly via specific glucose transporter(GLUT). In this study, the glucose transporter in mesangial cells was characterized by western blot and northern blot analyses. The western blotting using monoclonal antibodies against GLUTs 1 and 4 showed the presence of GLUT 1 but failed to detect GLUT 4. The northern blot analysis using cDNAs for GLUT 1,2,3,4,and 5 revealed the presence of GLUT 1 mRNA only, which was compatible with the results of western blotting. Furthermore, insulin-like growth factor 1 (IGF-1), which was able to increase the glucose uptake in mesangial cells, was found to stimulate the gene expression of GLUT 1. Since the mesangial cells possesses an abundant amount of receptors for IGF-1 and the IGF-1 levels in renal tissue is increased in diabetic state, the amount of glucose transport in mesangial cells may be regulated by IGF-1 and be increased in diabetes mellitus. In summary, GLUT 1 is a main glucose transporter in mesangial cells, regulated by IGF-1, and may play a role in the evolution of metabolic derangements found in diabetes mellitus.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 梶原 信之,吉川 隆一,羽田 勝計,古家 大祐,戸川 雅樹,堀出 直樹,宇津 貴,杉本 俊郎,繁田 幸: "培養ラットメサンギウム細胞のブドウ糖輸送体機構の研究" 日本腎臓学会誌. 33. 1287 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Haneda,R.Kikkawa,M.Togawa,D.Koya,N.Kajiwara,Y.Shigeta: "Metabolic of insulin-like growth factor 1 in cultured glomerular mesangial cells" Metabolism. 40. 1311-1316 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] R.Kikkawa,N.Kajiwara,M.Haneda,Y.Shigeta,K.Tsuda,H.Yano,Y.Seino: "GLUT 1 is a main glucose transporter in rat mesangial cells" J Am Soc Nephrol. 3. 830 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 前田 士郎,吉川 隆一,羽田 勝計,杉本 俊郎,古家 大祐,梶原 信之,宇津 貴,繁田 幸男: "ラット培養メサンギウム細胞における糖輸送担体(GLUTI)の調設機構" 日本腎臓学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masakazu Haneda, Ryuichi Kikkawa, Masaki Togawa, Daisuke Koya, Nobuyuki Kajiwara, Yukio Shigeta.: "Metabolic actions of insulin-like growth factor 1 in cultured glomerular mesangial cells." Metabolism. 40. 1311-1316 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ryuichi Kikkawa, Masakazu Haneda, Masaki Togawa, Daisuke Koya, Nobuyuki Kajiwara, Yukio Shigeta.: "Differential modulation of mitogennic and metabolic actions of insulin-like growth factor 1 in rat glomerular mesangial cells in high glucose culture." Diabetologia.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 梶原 信之,吉川 隆一,羽田 勝計 古家 大祐,戸川 雅樹,堀出 直樹 宇津 貴,杉本 俊郎,繁田 幸男: "培養ラットメサンギウム細胞のブドウ糖輸送体機構の研究" 日本腎臓学会誌. 33. 1287- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Haneda, R.Kikkawa, M.Togawa, D.Koya, N.Kajiwara, Y.Shigeta: "Metabolic actions of insulin-like growth factor 1 in cultured glomerular mesangial cells" Metabolism. 40. 1311-1316 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Kikkawa, N.Kajiwara, M.Haneda, Y.Shigeta, K.Tsuda, H.Yano, Y.Seino: "GLUT 1 is a main glucose transporter in rat mesangial cells" J Am Soc Nephrol. 3. 830- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 前田 士郎,吉川 隆一,羽田 勝計 杉本 俊郎,古家 大祐,梶原 信之 宇津 貴,繁田 幸男: "ラット培養メサンギウム細胞における糖輸送担体(GLUTI)の調節構構" 日本腎臓学会誌.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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