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Cloning of protein tyrosine phosphatase gene family and its gene expression

Research Project

Project/Area Number 03671161
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionWakayama Medical College

Principal Investigator

NISHI Masahiro  Wakayama Medical College, 1st Dept. of Medicine, Assistant Prof., 第一内科, 助手 (90228148)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsTyrosine phosphatase / Diabetes Mellitus / Gene expression / Cloning / チロシンホスファタ-ゼ / クロ-ニング
Research Abstract

1. Cloning of a tyrosine phosphatase(Leukocyte common antigen-related phosphatase : LRP) cDNA
Human LRP cDNA were cloned from a human kidney cDNA library by PCR. Human LRP consisted from 793 amino acids, and it contains transmembrane region and two phosphatase catalytic domains, which suggest it is a receptor type phosphatase.
2. Cloning of human LRP gene
Using human LRP cDNA as a probe, we screened human genomic library, and obtained several positive clones. Further analysis revealed that these clones did not cover the 5'end of cDNA. We are continuing the screening of the clones contains the 5'end of the LRP cDNA.
3. Gene expression of LRP
By RT-PCR method, we cloned partial cDNA of rat LRP. Using it as a probe, LRP gene expression are analyzed by Northern method. LRP gene expression are observed in brain, lung, liver, kidney, spleen, stomach, small and large intestine, skeletal muscle, heart, and pancreas. LRP gene expression in brain, liver, kidney, and skeletal muscle, are unchanged by fasting, refeeding, strepto-zotocin- or dexamethasone-induced hyperglycemia. These data suggest that expression of LRP may not correlate with hyperglycemia.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shinya Ohagi: "Sequence of a cDNA ercoding human LRP(Leukocyte Lommon antigen-related peptide)" Nucleic Acids Research. 18. 7159-7159 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 西 理宏: "新しい遺伝子ファミリーとしてのチロシンホスファターゼ" 実験医学. 9. 150-153 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 西 理宏: "PCR法を用いたチロシンホスファターゼのクローニング" 日本臨床代謝学会記録. 28. 106-107 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 西 理宏: "PCR法を利用したProtein Tyrosine Phosphatase遺伝子のクローニング" 分子糖尿病学. 3. 1-6 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shinya Ohagi: "Sequence of a cDNA emcoding human LRP(Leukocyte common autigen-related poptide)" Nucleic Acids Research. 18. 7159 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masahiro Nishi: "Cloning of tyrosine phosphatases by PCR" Proceedings of the Japan Society of Clinical Biochemistry and Metabolism. 28. 106-107 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masahiro Nishi: "Tyrosine phosphatase as a new gene family" Experimental Medicine. 9. 150-153 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masahiro Nishi: "Cloning of tyrosine phosphatase by PCR" Molecular diabetology. 3. 1-6 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shinya Ohagi: "Sequence of a cDNA encoding human LRP(Leukocyte common anfigen-related peptide)" Nucleic Acids Research. 18. 7159-7159 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] 西 理宏: "PCR法を用いたチロシンホスファターゼのクローニング" 日本臨床代謝学会記録. 28. 106-107 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 西 理宏: "PCRを利用したProtein Tynosine Phosphatase遺伝子のクローニング" 分子糖尿病学. 3. 1-6 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 西 理宏: "新しい遺伝子ファミリーとしてのチロシンホスファターゼ" 実験医学. 9. 150-153 (1991)

    • Related Report
      1992 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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