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pathophysiological significance of insulin-like growth factor binding protein

Research Project

Project/Area Number 03671168
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionTokyo Women's Medical Collage

Principal Investigator

TAKANO Kazue  Tokyo Women's Medical College, Dept of Medicine Associate Professor, 医学部, 助教授 (50096608)

Co-Investigator(Kenkyū-buntansha) ASAKAWA-YASUMOTO Kumiko  Tokyo Women's Medical College Department of Medicine Clinical Associate, 医学部, 助手 (80151017)
HIZUKA naomi  Tokyo Women's Medical College, Dept of Medicine Associate Professor, 医学部, 助教授 (80147397)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsinsulin-luke growth factor I(IGF-I) / IGF-II / IGF binding protein(IGFBP) / nonilet cell tumor associated with hypoglycemia(NICTH) / インスリン様成長因子II(IGF-I) / インスリン様成長因子結合蛋白(IGFBP / インスリン様成長因子I(IGFーI) / インスリン様成長因子II(IGFーII) / インスリン様成長因子(IGF)結合蛋白 / 低血糖
Research Abstract

Insulin-like growth factor(IGF)in serum and tissue binds to specific binding proteins. We investigated serum IGF binding proteins(IGFBPs)in various conditions using Western ligand blotting., Western immunoblotting of IGFBP-3 and RIA of IGFBP-1.
Serum from normal subjects revealed four IGFBPs with mol wts of 41/38, 34, 30, 24KD identified as IGFBP-3, 2, 1, 4 using Western ligand blotting. Serum IGFBP-3 decreased in the patients with GH deficiency and increased in acromegalic patients. IGFBP-3 sightly increased in patients with hypothyroidism and IGFBP-3 decreased and IGFBP-2 increased in malnutrition. All IGFBPs decreased in patients with severe liver dysfunction. These data show that serum IGFBP-3 was produced from liver and depends on GH and nutritional state. In patients with nonislet cell tumor associated with hypoglycemia(NICTH), serum IGFBP-2 increased. In two patients with NICTH IGFBP-2 decreased and IGFBP-3 increased after successful tumor resection.
Serum IGFBP-3 was also studied using Western immunoblotting. Serum from normal subjects revealed three bands of IGFBP-3 with mol wts of 41, 38, 30KD. These three bands of IGFBP-3 increased in acromegalic patients and decreased in patients with GH deficiency. Serum in pregnant wemen shows a large amount of 30KD IGFBP-3. In some patients with NICTH most of all IGFBP-3 was 30KD and this band disappeared after successful tumor resection.
When 0.06mg/kg or 0.12mg/kg IGF-I was administered to 5 normal subjects, IGFBP-1, which was measured using RIA, increased at 1h and reached the peak at 5hs.
We reported serum IGFBP in various conditions. These observations are useful for studying IGF bioavailability.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Takano K.et al.: "Repeated sc admiristration of secomfinant human insulin-like gwooth factor I(IGF-I)to haman subjicts for 7 days." Growth Regulation. 1. 23-28 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hizuka N.et al.: "Measusement of free form of insulin-like growth factor I in human plasma." Growth Regulation. 1. 51-55 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tanaka T.et al.: "Growth hormone secretion and therapeutic effects of human growth hnmone:First Gaparse rsults of kabi Phormacic international growth study/international woperatiae growth study." Acta Paediatr Scand.379. 126-135 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano K.et al.: "Treatment of 46 patients with Turneis syndrome with recomfinant human growth hormore(YM-17798)for three years.-Malticentre study-" Act a Endo uipologica. 126. 296-302 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Asakawa-Yasumoto K.et al.: "Effects of insulin-like growth factan I or human growth hormre in fosted rats." Growth Regulation. 2. 40-44 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano K,et al.: "Turner syndrome;Growth promoting therapies" Ranke M.B.& Rosenfeld R.G., 249-255 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hizuka N.et al.: "Modern concepts of insulin-like growth factors" Spencer E.M., 51-55 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano K. et al.: "Treatment of 46 patients with Turner′s syndrome with recombin ant human growth hormone(YM-17798)for three yeas. -Multi contre study-" A ct a Endocripologica. 126. 296-302 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Asakawa-Yasumoto K. et al.: "Effects of insulin-like growth factor Ior human growth hormone in fasted rats." Growth Regulation. 2. 40-44 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takan K.et al.: "Repeated SC administration of re combinant Auman insulinーlike gnewith factor I(IGFーI)to human subijects for 7 days." Growth Regulation. 1. 23-28 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高野 加寿恵 他: "成人下垂体性小人症における遺伝子組換えヒト型IGFーI(FK780)単回及び連日7日間投与の安定性および血中動態の検討" 薬理と治療. 20. 557-567 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hizuka N.et al.: "Measure ment of free form of insulinーlike growth factor I in human plosma." Growth Regulation. 1. 51-55 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takano K.et al.: "Turner syndrome;Growth promoting the rapies" Ranke M.B&Rosenfeld R.G., 249-255 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hizuka N.et al.: "Modern concepts of insulinーlike growth factors" Spencer E.M., 51-55 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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