pathophysiological significance of insulin-like growth factor binding protein
| Project/Area Number |
03671168
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Tokyo Women's Medical Collage |
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Principal Investigator |
TAKANO Kazue Tokyo Women's Medical College, Dept of Medicine Associate Professor, 医学部, 助教授 (50096608)
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| Co-Investigator(Kenkyū-buntansha) |
ASAKAWA-YASUMOTO Kumiko Tokyo Women's Medical College Department of Medicine Clinical Associate, 医学部, 助手 (80151017)
HIZUKA naomi Tokyo Women's Medical College, Dept of Medicine Associate Professor, 医学部, 助教授 (80147397)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | insulin-luke growth factor I(IGF-I) / IGF-II / IGF binding protein(IGFBP) / nonilet cell tumor associated with hypoglycemia(NICTH) / インスリン様成長因子II(IGF-I) / インスリン様成長因子結合蛋白(IGFBP / インスリン様成長因子I(IGFーI) / インスリン様成長因子II(IGFーII) / インスリン様成長因子(IGF)結合蛋白 / 低血糖 |
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| Research Abstract |
Insulin-like growth factor(IGF)in serum and tissue binds to specific binding proteins. We investigated serum IGF binding proteins(IGFBPs)in various conditions using Western ligand blotting., Western immunoblotting of IGFBP-3 and RIA of IGFBP-1.
Serum from normal subjects revealed four IGFBPs with mol wts of 41/38, 34, 30, 24KD identified as IGFBP-3, 2, 1, 4 using Western ligand blotting. Serum IGFBP-3 decreased in the patients with GH deficiency and increased in acromegalic patients. IGFBP-3 sightly increased in patients with hypothyroidism and IGFBP-3 decreased and IGFBP-2 increased in malnutrition. All IGFBPs decreased in patients with severe liver dysfunction. These data show that serum IGFBP-3 was produced from liver and depends on GH and nutritional state. In patients with nonislet cell tumor associated with hypoglycemia(NICTH), serum IGFBP-2 increased. In two patients with NICTH IGFBP-2 decreased and IGFBP-3 increased after successful tumor resection.
Serum IGFBP-3 was also studied using Western immunoblotting. Serum from normal subjects revealed three bands of IGFBP-3 with mol wts of 41, 38, 30KD. These three bands of IGFBP-3 increased in acromegalic patients and decreased in patients with GH deficiency. Serum in pregnant wemen shows a large amount of 30KD IGFBP-3. In some patients with NICTH most of all IGFBP-3 was 30KD and this band disappeared after successful tumor resection.
When 0.06mg/kg or 0.12mg/kg IGF-I was administered to 5 normal subjects, IGFBP-1, which was measured using RIA, increased at 1h and reached the peak at 5hs.
We reported serum IGFBP in various conditions. These observations are useful for studying IGF bioavailability.
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Report
(3 results)
Research Products
(14 results)
