| Project/Area Number |
03671174
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Institute of Clinical Medicine, University of Tsukuba |
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Principal Investigator |
NAGASAWA Tosihrou Institute of Clinical Medicine, assistant University of Tsukuba professor, 臨床医学系, 講師 (70014298)
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| Co-Investigator(Kenkyū-buntansha) |
三井 洋司 微生物工業技術研究所, 動物細胞研究室, 首席研究官
MITSUI Y National Institute of chief Bioscience & Human-Technology investigation of Indus
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | endothelial cells / megakaryocytes / megakaryocyte maturating factor / platelet / interleukin-1 / 巨核球系細胞株 |
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| Research Abstract |
We have designed following experiments to determine whether endothelial cells (EC) were capable of maturating megakaryocytes. 1)Do human umbilical vein endothelial cells(HUVEC) maturate human megakaryocyte cell line(CMK-7)?, 2)Do HUVEC maturated human megakaryocytes separate from bone marrow?, 3)Do HUVEC stimulate by thrombocytopenic serum may generate platelet-releasing factor? CMK-7 overlaid on the HUVEC increased cell-size, and content of ADP and serotonin. Also CMK-7 developed cytoplasmic projection with proplatelet formation. These findings were observed in a direct contact culture. In next, human megakaryocytes separated by Celsep separation system developed well cytoplasmic projections on the HUVEC monolayer. These phenomenon was only observed in a contact with megakaryocyte and HUVEC. Furthermore, megakaryocytes cultured on the HUVEC treated with platelet lysate developed many cytoplasmic processes with proplatelet formation. These data suggest an interaction between megakaryocyte and HUVEC give an important role for the terminal maturation of megakaryocyte. Especially, adhesion mechanism between megakaryocyte and HUVEC should be further investigated for the platelet production of magakaryocytes.
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