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Signal transduction and products of oncogenes in megakaryoblastic leukemia cells

Research Project

Project/Area Number 03671175
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionChiba university

Principal Investigator

SATO Takeyuki  Chiba University, School of Medicine, Director, 医学部, 講師 (30187207)

Co-Investigator(Kenkyū-buntansha) KAKUDA Harumi  National Cancer Center, EAST HOSPITAL, Doctor, 厚生技官
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsCMK cells / Retinoic acid / c-raf / c-fos / c-myc / Signal transduction / oncogenes / PDGF(Platelet derived growth factor) / c-raf / c-fos / c-myc / PDGF(血小枝由来増殖因子) / cーraf
Research Abstract

All trans retinoic acid(RA) enhanced the expression of craf mRNA in the megakaryoblastic leukemia cell line, CMK. Platelet derived growth factor(PDGF) also enhanced it, but the amount of it is small. IL-3, IL-6, GM-CSF, G-CSF, erythropoietin, phorbol ester(TPA), DMSO and gamma-interferon didn't show any change.
The expression of c-raf mRNA was initially increased by the treatment of RA, but strongly suppressed after 4 weeks of treatment. Stimulation by fetal calf serum(FCS) to the CMK cells, which were strongly suppressed the expression of c-raf mRNA by RA, induced the decrease in intensity of expression of c-fos mRNA compared with untreated cells. The expression of c-fos mRNA reached the maximal level at 15-30 minutes after FCS treatment in both cells. There was no difference of the expression of c-myc mRNA between these cells. This result indicated that raf gene may be related the signal transduction to fos gene not to myc gene.
Treatment of CMK cells by TPA intensively increased the expression of small GTP-binding protein, smg p21. The study of the relationship between the expression of c-raf gene and smg p21 gene is on going. CMK cells were induced the proliferation and/or differentiation by GM-CSF, IL-3, IL-6, and IL-11. The study of effects of these cytokines on CMK cells which were suppressed the expression of c-raf mRNA is also on going.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] NAGANO,T.: "Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line(CMK11-5)." Int.J.Hematol.56. 67-78 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ADACHI,M.: "Induction of smy q21/rap1A p21 geneexpression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line." Oncogene. 7. 323-329 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] IIDA,S.: "Human hst-2(FGF-6)oncogene:cDNAcloning and characterization." Oncogene. 7. 303-309 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KATOH,O.: "Expression of the heparin-binding growth factor receptor gene in human megakaryocytic leukemia cells." Biochem.Biophys.Res.Comm.183. 83-92 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] AKIYAMA,Y.: "Tumor necrosis factor-a stimulates colony formation by a megakaryoblastic leukemia cell line,CMK." Jan.J.Cancer Res.83. 989-994 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MIURA,K.: "Stimulatory effect of tumor necrosis factor-a on the growth of CMK,a human megakaryoblastic leukemia cell line." Leukemia Res.16. 281-285 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nagano, T. et al.: "Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line (CMK11-5)." Int. J. Hematol.56. 67-78 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Adachi, M. et al.: "Induction of smg p21/rap1A p21/krev-1 p21 gene expression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line." Oncogene,. 7. 323-329 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Iida, S et al.: "Human hst-2(FGF-6)oncogene:cDNAcloning and characterization." Oncogene,. 7. 303-309 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katoh, O. et al.: "Expression of the heparin-binding growth factor receptor gene in human megakaryocytic leukemia cells." BBRC. 183. 83-92 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Akiyama, Y. et al.: "Tumor necrosis factor-alpha stimulates colony formation by a megakaryoblastic leukemia cell line, CMK." Jpn. J. Cancer Res.83. 989-994 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Miura, K. et al.: "Stimulatory effect of tumor necrosis factor-alpha on the growth of CMK, a human megakaryoblastic leukemia cell line." Leukemia Res.16. 281-285 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nagano,T.: "Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line (CMK11-5)." Int.J.Hematol.56. 67-78 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Adachi,M.: "Induction of smg p21/rap1A p21/krev-1 p21 gene expression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line." Oncogene. 7. 323-329 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Iida,S.: "Human hst-2(FGF-6)oncogene:cDNAcloning and characterization." Oncogene. 7. 303-309 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh,O.: "Expression of the heparin-binding growth factor receptor gene in human megakaryocytic leukemia cells." Biochem.Biophys.Res.Comm.183. 83-92 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Akiyama,Y.: "Tumor necrosis factor-α stimulates colony formation by a megakaryoblastic leukemia cell line,CMK." Jpn.J.Cancer Res.83. 989-994 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miura,K.: "Stimulatory effect of tumor necrosis factor-α on the growth of CMK,a human megakaryoblastic leukemia cell line." Leukemia Res.16. 281-285 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fuse A,Kakuda H,Shima Y,Dame JV,Billiau A and Sato T: "Interleukin6,a pssible autocrine growth and differentiation factor for the human megakaryocytic cell line,CMK" British Journal of Haematology. 77. 32-36 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakaguch M,Sato T and Groopman JE: "Human immunodeficiency virus infection of megakaryocytic cells" Blood. 77. 481-485 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iida S,Yoshida T,Naito K,sakamoto H,Katoh O,Hirohashi S,Sato T,Onda M,Sugimura T,Terada M: "Human hstー2 oncogene:cDNA cloning and characterization" Oncogene.

    • Related Report
      1991 Annual Research Report
  • [Publications] Nagano T,Ohga S,Kishimoto Y,Kimura T,Yasunaga K,Adachi M,Ryo R,Sato T: "Ultrastructural analysis of platelatーlike particles from a human megakaryocytic leukemia cell line(CMK)" International Journal of Hematology.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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