| Project/Area Number |
03671181
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKAMURA Shinobu Kanazawa Univ. School of Med. Associate prof., 医学部, 助教授 (20019946)
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| Co-Investigator(Kenkyū-buntansha) |
RYOYAMA Kazuo Kanazawa Univ. Cancer Res. Inst. Lecturer, がん研究所, 助手 (50019874)
NOMURA Takahiro Kanazawa Univ. Cancer Res. Inst. Lecturer, がん研究所, 助手 (80115261)
HOSONO Ryuji Kanazawa Univ. School of Med. Lecturer, 医学部, 講師 (40019617)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | SFME cell / Oncogene / Hematologic malignancy |
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| Research Abstract |
1. Objective: In this study, it has been attempted to establish an useful method for monitoring oncogenes in hematologic and other malignancies using Serum-Free Mouse Embryo (SFME) cells which are readily transformed by human cancer cells-derived proto-oncogenes.
2. Establishment of culture condition of SFME cells: First, in order to obtain the best culture condition for SFME cells, the cells were cultured under various conditions. It became clear that it is essential to use purified water for cultures and to add various additives (EGF etc.) at the time of medium exchange. Furthermore, a glass apparatus is not suitable for culturing SFME.
3. Tumorigenicity of H-ras and c-myc proto-oncogenes-transformed SFME cells: It was confined that transformed SFME cells are transplantable to syngeneic mouse (BALB/C), and are able to develop tumor in mouse.
4. Characteristics of transformed SFME cells: Transformed SFME cells were injected subcutaneously into BALB/C mouse and patterns of involvement in the various organs were observed sequentially. Consequently,a transformed cell line which frequently metastasize to the lung (r/mHM-SFME-1) were established. In an attempt to develop a gene-monitoring system, numbers of metastasized r/mHM-SFME-1 cells in the lung of BALB/C were analyzed using PCR method. The detectable minimum number of metastasized cells in the lung per mouse was 1X10^4 cells, and there was a linear correlation between the amount of DNA and a number of metastasized cell.
5. Application of this system for oncogene monitor in hematologic malignancies: Analizing SFME cells transformed by human leukemic cells-derived proto-oncogene make it possible to evaluate chemotherapeutic effects and to detect minimal residual disease readily.
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