| Project/Area Number |
03680136
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Chiba University |
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Principal Investigator |
KIMURA Sadao Chiba University, School of Medicine, Professor, 医学部, 教授 (40134225)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | endothelin / big endothelin / endothelin converting enzyme / phosphoramidon / ACHN細胞 |
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| Research Abstract |
1. Two phosphoramidon sensitive-neutral metalproteases (M1 and M2) with endothelin converting activity were detected in membranous fractions of porcine lung. M1 specifically converts only big ET-1 to ET-1 not big ET-2 and big ET-3 to ET-2 and ET-3, respectively. Taking together with other biochemical analyses, it is suggested that M1 is a new physiological enzyme and M2 may be a known neutral endopeptidase
2. (i) Analysis of endothelin-2 (ET-2) biosynthetic pathway showed that ET-2 is produced via big ET-2(1-38) as an intermediate form. The biochemical characterization of ET-2 converting enzyme (ECE-2) indicates that ECE-2 is a phosphoramidon-sensitive metal protease and very similar to endothelin-1 converting enzyme (ECE-1) from endothelial cells.
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