| Project/Area Number |
03680216
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
ISONO Kunio TOHOKU UNIVERSITY GRADUATE SCHOOL OF INFORMATION SCIENCES, 大学院・情報科学研究科, 助手 (70124550)
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| Co-Investigator(Kenkyū-buntansha) |
SIMADA Ichiro TOHOKU UNIVERSITY SCIENCES PROFESSOR, 理学部, 教授 (50108429)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | DROSOPHILA / TASTE / MUTANT / MOLECULAR BIOLOGY / PHYSIOLOGY / Drosophila / taste mutation / taste receptor |
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| Research Abstract |
In vivo expression system using Xenopus oocyte by injecting polyA or total RNA extracted from labellar taste organs of the fleshfly has been established. An inward current was observed when the egg was perf used with sugar stimulants that stimulate fly taste receptors. In most cases the response was much higher for sugars like sucrose or glucose and smaller or absent for non-stimulating sugars like mannose or lactose.
Genomic fragments that possibly cover the Drosophila taste gene Tre were isolated from YAC clones. Since the fragment contains more than 100 Kb length we are currently subcloning smaller fragments to identify the gene.
The effect of Tre were analyzed physiologically by recording the labellar sensory neurons and compared with behavioral results that had been published earlier.
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