| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
The silkworm, Bombyx mori, experimental material was a hybrid of CTKXCambodge(heterozygous normally pigmented egg and normal color larva:+/re,+/ch). The ovarian eggs of the CTK X Cambodge were treated with centrifugation, acid, ultravox, low-temperature, cold shock and hot-water. The effect of meiotic parthenogenesis was examined using the percentage of red pigmented eggs(re) as indices.
The eggs of spontaneous and artificial parthenogenesis showes a normal or red pigmented in the ratio of 1:1. The eggs of spontaneous parthenogenesis and artificial partheneogensis induced by centrifugation, ultravox, low-temperature were few. Above 20% of the treated eggs with cold shock treatment(-11ﾟC, 30min), acid-treatment (HCl;S.G. 1.050, 40ﾟC, 20min) and hot-water treatment(46ﾟC, 2min) manifested meiotic parthenogenetic eggs. Hot-water treatment was easier than other methods, and ameiotic parthenogenesis was induced by 46ﾟC,18min treatment. So hot-water treatment was examined in detail. Meiotic parthenogenetic eggs were appeared at a high rate, when the ovarian eggs were kept at 17-25ﾟC before and after the treatment. Normal larvae(+ch) and chololate larvae(ch) were hatched out from the eggs of meiotic parthenogenesis.
But, the hatchability of these meiotic parthenogic eggs was very low, less than 1%, irrespective of the treatments applied. Polyploidy in embryos of the meiotic parthenogenesis was estimate by flow cytometry. The mixoploid was the n+2n+3n mixoploid type.
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