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Analysis of Antitumor Glycopeptide Containing Sialic Acid At High Level Obtained From Hen Egg White

Research Project

Project/Area Number 03806042
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産化学
Research InstitutionGifu University

Principal Investigator

WATANABE Kenji  Gifu University, Faculty of Agriculture, Professor, 農学部, 教授 (70023447)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsHen Egg Thick White Protein / beta-Ovomucin / Antitumor Activity / Sialoglycoprotein / 鶏卵濃厚卵白 / βーオボムチン / 鶏卵農厚卵白
Research Abstract

Glycoprotein (HSGT.molecular weight :400kDa) containing sialic acid at high level was fractionated by the general method from ovomucin in the thick egg white of fresh hen egg. and then sialoglycopeptide (HSGPE) was also fractionated by the methods of gel-filtration and affinity chromatography from the HSGT digested with trypsin. When the tumor cells such as Melanoma cell. Lewis lung cancer cell. Heptoma cell. SR180 cell and the like were incubated with the prepared samples. the clear decrease or evanescence of microvilli and the definitive swelling like petal of microvilli were observed with scanning electron microscope. with the increasings of the added amounts of samples and the incubation time. Moreover. when the described-above tumor cells were also incubated with the same prepared samples in the presence of 6-^3H-tymidine to determine their inhibitory activity for the synthesis of radioactive nucleotides. the decreasings of DNA synthesis in their cells were observed at the decreased levels of 15-40%. comparing with the case of no addition of samplces. The decreased ratios were different every tumor cells. These results indicated that the sensibility of the cells against samples was different. On the other hand. the morphological change in the normal cells incubated with the prepared samples was not observed. Thus. HSGT and HSGPE were found to have antitumor activities for the tumor cells.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 渡邊乾二: "乳の感染防御因子" 乳技協資料. 42. 1-14 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 大網弘: "鶏卵卵白オボムチン由来シアロ糖蛋白質の腫瘍細胞傷害作用" 医学と生物学. 126. 19-23 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 渡邊乾二: "Functions of Fermented Milk" Elsevier Applied Science, 21 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 渡邊乾二: "ミルクのサイエンス" 全国農協乳業プラント協会, 16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji Watanabe: "Antiinfective factors in milk" Bulletin Jap. Dairy Tech. Asso. 42. 1-14 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroshi Ohami: "Cytotoxic effect of sialoglycoprotein derived from avian egg white ovomucin on the cultured tumor cell." Medicine and Biology. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji Watanabe: "Functions of fermented milk." Elsevier Applied Science. 355-376 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji Watanabe: "Science of milk" NAMP Assoc. 121-137 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 渡邉 乾二: "乳の感染防御因子" 乳技協資料. 42. 1-14 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 大網 弘: "鶏卵卵白オボムチン由来シアロ糖蛋白質の腫瘍細胞傷害作用" 医学と生物学. 126. 19-23 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 渡邉 乾二: "ミルクのサイエンスII" 全国農協乳業プラント協会, 16 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 渡邉 乾二: "畜産大辞典" 養賢堂, 10 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 大網 弘: "鶏卵卵白オボムチン由来シアロ糖蛋白質の培養腫瘍細胞傷害作用ー細胞の形態学的変化ー" 医学のあゆみ. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 横田 隆: "鶏卵卵白オボムチン由来シアロ糖蛋白質の腫瘍細胞DNA生合成の阻害" 癌治療学誌. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 中村 良,川岸 舜朗: "食品分析学" 文永堂出版, 285 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 細野 明義: "ミルクの新しい働き" 全国農協乳業プラント協会, 130 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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