| Project/Area Number |
03807009
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OYAMA Yasuo The University of Tokushima,Fac.of Integrated Arts&Sciences Associate Professor, 総合科学部, 助教授 (80214229)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Neurotoxicity / Triorganotin / Triphenyltin / Tributyltin / Cytosol calcium / Ca permeability / Brain neuron / Ionic channels / 中枢神経細胞 / 単離細胞 / 電圧固定法 / ナトリウム電流 / 光化学測定法 / 細胞内カルシウム / 有機錫 |
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| Research Abstract |
Effects of triphenyltin were examined on dissociated rat brain neurons and mouse thymocytes using patch clamp technique and flow-cytometric technique. Triphenyltin increased the current amplitude of voltage-dependent Na^+ current by inhibiting the inactivation process,associated with decreasing the voltage-dependent K^+ currents(delayed and transient K^+ currents),in brain neurons. Futhermore, triphenyltin increased the intracellular Ca^<2+> concentration by increasing the membrane Ca^<2+> permeability and releasing Ca^<2+> from the intracellular calcium stores in both brain neurons and thymocytes. Therefore,triphenyltin may cause hyperexcitability on mammalian CNS. Tributyltin possessed similar actions on the intracellular Ca^<2+> concentration while it was not the case for diphenyltin,monophenyltin, dibutyltin and monobutyltin.
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