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Atrial Natriuretic Peptide Receptors on the Glomerular Epithelial Cell and Their Roles in Regulation of Glomerular Ultrafiltration

Research Project

Project/Area Number 03807018
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNIIGATA UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

YAMAMOTO Tadashi  NIIGATA UNIVERSITY SCHOOL OF MEDICINE, Associate professor, 医学部, 助教授 (30092737)

Co-Investigator(Kenkyū-buntansha) KAWASAKI Katsutoshi  NIIGATA UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (20152944)
KIHARA Itaru  NIIGATA UNIVERSITY SCHOOL OF MEDICINE, Professor, 医学部, 教授 (80018324)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
KeywordsGlomerular epithelial cell / Atrial natriuretic peptide / Glomerular ultrafiltration / Receptor / mRNA / 限外瀘過調
Research Abstract

Major effects of atrial natriuretic peptide (ANP) on the kidney are diuresis and natriuresis. The ANP-induced diuresis is caused by an increase of glomerular filtration rate. To understand how ANP increases glomerular filtration rate, the receptor for ANP have been localized in the kidney. The receptor was shown in the glomeruli and inner medulla by ligand binding experiments. However, recent molecular biology techniques disclosed the presence of three distinct subtypes of ANP receptors; two biological receptors (A and B) and a clearance receptor (C). In the present study, complementary RNA probes which discriminate these receptor subtypes were used to localize them individually.
Ribonuclease protection assay demonstrated that mRNA for type A and type C ANP receptors were predominantly present in the glomeruli and mRNA for type A in the inner medulla. The type C receptor mRNA was found exclusively in the glomerulus and the alternative splicing of the type C mRNA was suggested by the ribonuclease protection assay. By in situ hybridization, the type A receptor mRNA was localized in the glomerular epithelial cells and the epithelial cells of inner medullary collecting tubules. These results indicate that ANP excites the glomerular epithelial cell through type A ANP receptors to increase ultrafiltration coefficient and subsequently glomerular filtration rate and propose a hypothesis that the glomerular epithelial cell plays an important role in the regulation of glomerular filtration rate.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tadashi YAMAMOTO: "Effet of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat." Kidney International. 40. 705-713 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi KAWASAKI: "Depletion of CD8 positive cells prevents proteinuria and glomerular injury in nephrotoxic serum nephrtis" Kidney International. 41. 1517-1526 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi KAWASAKI: "Antibodies against intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 prevent glomerular injury in rat" J Immunology. 150. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Roland C.Blantz: "The role of the mesangial cell in the glomerular response to volume and angiotensin II." Am J Physiol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi YAMAMOTO: "Expression of mRNA for atrial natriuretic peptide receptor subtypes in the kidney" in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi YAMAMOTO: "Immunohistochemecal localization of atrial natriuretic peptide type C receptor in the rat organs" in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Atrial natriuretic peptide receptor subtypes in the kidney" Nisimura Co.Ltd,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat." Kidney Int. 40. 705-713 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi Kawasaki: "Depletion of CD8 positive cells in nephrotoxic serum nephritis of WKY rats." Kidney Int. 41. 1517-1526 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katsutoshi Kawasaki: "Antibodies against intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 prevent glomerular injury in rat experimental crescentic glomerulonephritis. ." J Immunol. 150. 1074-1083 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Roland C. Blantz: "The role of mesangial cell in the glomerular response to volume and angiotensin II." Am J Physiol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Expression of mRNA for atrial natriuretic peptide receptor subtypes in the kidney."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Immunohistochemical localization of atrial natriuretic peptide type C receptor in the rat organs."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Atrial natriuretic peptide receptor subtypes in the kidney. Ed. by Arakawa M and Gejo F in Morphological, Immunological and Biological Approach to Glomerular Injuries. Nishimura Co. Ltd."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tadashi Yamamoto: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat." Kidney International. 40. 705-713 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katsutoshi Kawasaki: "Depletion of CD8 positive cells prevents proteinuria and glomerular injury in nephrotoxic serum nephritis" Kidney International. 41. 1517-1526 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katsutoshi Kawasaki: "Antibodies against intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 prevent glomerular injury in rat" Journal of Immunology. 150. 1074-1083 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yutaka Matsuki: "Detection of inflammatory cytokine messenger RNA-expressing cells in human gingiva by combined in situ hybridization and immuno-histochemistry" Immunology. 76. 42-47 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sentaro Kobayashi: "The molecular biology study of the expression of thrombospondin in vascular smooth muscle cells and mesangial cells" Journal of Diabetic Complications. 5. 121-123 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Roland C. Blantz: "The role of mesangial cell in the glomerular response to volume and angiotensin II" American Journal of Physiology.

    • Related Report
      1992 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Atrial natriuretic peptide receptor subtypes in the kidney, in Molphological, Immunological and Biological Approach to Glomerular Injuries" Nishimura Co. Ltd, 67-78 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Effect of mesangial cell lysis and proliferation on glomerular hemodynamics in the rat." Kidney International. 40. 705-713 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Participation of cytokines in proliferation of mesangial cell." Acta Med.et Biol.39. 87-91 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Matsuki: "Interleukinー1 mRNAーexpressing macrophages in human chronically inflamed gingival tissues" Am.J.Pathol.138. 1299-1305 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Katsutoshi Kawasaki: "Depletion of CD8 positive cells prevents proteinuris and glomerular injury in nephrotixic serum nephritis" Kidney International.

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadashi Yamamoto: "Immunohistochemical localization and mRNA expression of atrial natriuretic peptide type C receptor in the Kidney"

    • Related Report
      1991 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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