| Project/Area Number |
03807048
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Nagoya University |
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Principal Investigator |
TANAKA Masashi Department of Biomedical Chemistry,Faculty of Medicine,University of Nagoya,Associate Professor, 医学部, 助教授 (60155166)
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| Co-Investigator(Kenkyū-buntansha) |
SAHASHI Ko Neurol.Section,Dept.of Med.,Aichi Med.Univ.,Associate Professor, 医学部, 助教授 (90131242)
OZAWA Takayuki Dept.of Biomed.Chem.,Fac.of Med.,Univ.of Nagoya,Professor, 医学部, 教授 (80022771)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Spinocerebellar degeneration / Mitochondria / Mitochondrial DNA / Ataxia / Nucleotide sequencing / Point mutation / Energy production / Ageing / 脊髄小脳変性症 / ミトコンドリア / ミトコンドリアDNA / 運動失調 / 塩基配列決定 / 遺伝子増幅 / エネルギー産生 / 加齢 / エネルギ-産生 |
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| Research Abstract |
Spinocerebellar degeneration represents a group of heterogeneous degenerative diseases showing ataxia as the main symptom. This disorder is clinically characterized by slowly progressive course,and various combinations of signs and symptoms of the pyramidal and extrapyramidal tracts and the peripheral nervous system. In order to investigate the possible role of mitochondrial dysfunction in the pathogenesis of extensive neuronal degeneration in this disorder,we have analyzed the mutations of mitochondrial DNA in patients with spinocerebellar degeneration. Mitochondrial DNA(mtDNA)was amplified by the polymerase chain reaction (PCR)from DNA isolated from platelets of each patient.The single strand DNA was amplified from the first PCR product by using the asymmetric PCR method. The second PCR product was used as the template for the fluorescence-based direct PCR sequencing. Sequences of these mtDNA fragments were analyzed by an automated DNA sequencer. In 43 cases of spinocerebellar degene
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ration,sequence analysis and restriction fragment analysis revealed neither the 8344 A-to-G nucleotide substitution reported in patients with MERRF syndrome nor the 8993 G-to-T transversion reported in a patient with cerebellar ataxia,retinitis pigmentosa, and cardiomyopathy. Sequence analysis of the whole mtDNA from two patients whose family histories were consistent with maternal inheritance revealed multiple mutations in the non-coding regions,rRNA genes,and mRNA genes. Although some of these mutations in these two patients were also observed in other disease and normal controls,several nonsynonymous mutations in Patient 2 were not found in the normal controls. Both of the mutations in the ND2 and ATP6 genes in Patient 2 were also found in a paient with Parkinson's disease as well as in Control 1,who died at the age of 55 from gastric cancer. The possible involvement of these mutations in the pathogenesis of spinocerebellar degeneration must be evaluated from further study on the sequence heterogeneity of mtDNA among normal controls as well as disease controls. Less
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