| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
Three human esophageal carcinoma cell lines (HPL-EsC-1-K, -S, and -M) were studied on their tumorigenic and metastatic properties in nude mice. All cell lines grew in the hind foot pads following subcutaneous inoculation and produced popliteal lymph node metastasis dose (2-8x10^6)-dependently. Based on the histopathological findings on serial sectins of the lymph nodes, the stages of lymph nodes invasion by cancer cells were classified into 4 stages (St.0-III). The time course of lymph node metastasis of EsC-K cells were examined. Advanced stage of metastasis increased according to the time elapsed after tumor cell inoculation. Incidence of metastasis of EsC-K cells were not affected by host factors such as sex differences, anti-asialo GM1 antibody treatment on the hosts.
Cellular adhesion to coated glycoproteins (5mug/cm^2 fibronectin, 2.5mug/cm^2 laminin, and 2.5mug/cm^2 typeIV collagen) was analysed. HPL-EsC-1-K, -S and -M were derived from same donor but exhibited heterogeneity of lymph node metastasis in nude mice (K:60%, S:80%, and M:40%). In binding assays of these sublines, the number of adhered cells were correlated to ratio of lymph node metastasis. HPL-EsC-3, that was xenotransplated but no metastasis to local lymph node was observed, highly adhered to the glycoproteins.
Flowcytometric analysis of expression of various adhesion molecules in esophageal cancer cell lines was performed using monoclonal antibodies. No apparent correlation between expression of adhesion molecules and lymph node metastasis in nude mice was noted.
Study on the effect of epidermal growth factor (EGF) on growth of xenotransplanted esophageal cancer (ES-29, ES-42, and ES-47) showed no apparent contribution of EGF to tumor growth.
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