Basic study and clinical application of genetic diagnosis for lung cancer using polymerase chain reaction (PCR).

• Project/Area Number: 03807088
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Tokyo Medical College
• Principal Investigator: KATO Harubumi (1992-1993) Tokyo Medical College, Dept.of Surgery, Professor, 医学部, 教授 (20074768); 中村 治彦 (1991) 東京医科大学, 医学部, 講師 (80183523)
• Co-Investigator(Kenkyū-buntansha): UEDA Shintaro Tokyo University, Science Faculty, Associate Professor, 理学部, 助教授 (20143357) ; NAKAMURA Haruhiko Tokyo Medical College, Medical Faculty, Assistant Professor, 医学部, 講師 (80183523) ; TAKAHASHI Hidenobu Tokyo Medical College, Medical Faculty, Assistant Professor, 医学部, 講師 (20201600) ; SAITO Makoto Tokyo Medical College, Medical Faculty, Assistant Professor, 医学部, 講師 (30225734) ; KONAKA Chimori Tokyo Medical College, Medical Faculty, Associate Professor, 医学部, 助教授 (70147180) ; 加藤 治文 東京医科大学, 医学部, 教授 (20074768)
• Project Period (FY): 1991 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
• Keywords: p53 / PCR / lung cancer / p53 / 遺伝子診断 / SSCP

Research Abstract

Recently it has been reported that the tumor suppressor gene p53 is frequently mutated in primary lung cancer and various other types of cancer. So we examined the p53 mutation pattern in lung cancer by the PCR-SSCP(polymerase Chain reaction-single strand conformation polymorphism), a simple and sensitive method for detection of DNA mutation. And we analyzed its relationship with clinicopathological features. Tumor specimens were obtained from primary lung cancer of 45 patients, who were treated surgically at the Tokyo Medical College Hospital, Tokyo. Because 98% of p53 gene mutation in different cancers have been found in exon 5-8, we focused our study on these exons. The oligonucleotide primers for amplification of exons 5-8 were designed based on the published sequence. Fifteen of the 45 (33%) lung cancer cases showed abnormally shifted bands on PCR-SSCP analysis of the p53. The percentage of p53 mutation-positive cases varied to the histological type. In adenocarcinoma the incidence of cases detected p53 mutation showed a tendency to increase with advancing pathological stage. This result indicate that mutation of the p53 gene plays an important role in the development of primary lung cancer and its a potential prognostic factor in adenocarcinoma of the lung. We consider that a careful follow-up is necessary for case detected mutation of p53 gene.

Research Products

• [Publications] Haruhisa Hiyoshi et al.: "Clinicopathological Significance of Nuclear Accumulation of Tumor Suppressor Gene p53 Product in Primary Lung Cancer" Japanese Journal of Cancer Reseach. 83. 101-106 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Haruhisa Hiyoshi, Yoshihiro Matsuno, Harubumi Kato, Yukio Shimosato and Setsuo Hirohasi: "Clinicopathological significance of nuclear accumulation of tumor suppressor gene p53 product in primary lung cancer." Jpn.J.Cancer Res.83. 101-106 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Haruhisa Hiyoshi et al.: "Clinicopathological Significance of Nuclear Accumulation of Tumor Suppressor Gene p53 Product in Primary Lung Cancer" Japanese Journal of Cancer Reaseach. 83. 101-106 (1992)
• [Publications] Haruhisa Hiyoshi et al.: "Clinicopathological Significance of Nuclear Accummlation of Tumor Supprssor Gene p53 Product in Primary Lung Cancer" Japanese Journal of Cancer Research. 83. 101-106 (1992)