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The effect of tumor necrosis factor-alpha on hypothalamopituitary -ovarian axis

Research Project

Project/Area Number 03807107
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

KOIKE Koji  Osaka Univ, Med.School Assistant Professor, 医学部, 助手 (70225340)

Co-Investigator(Kenkyū-buntansha) HIROTA Kenji  Osaka Univ, Med.School Lecturer, 医学部, 講師 (00189888)
IKEGAMI Hiromasa  Osaka Univ, Med.School Assistant Professor, 医学部, 助手 (10184409)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Keywordstumor necrosis factor-alpha / anterior pituitary / arachidonate / Hypothalamus / Paracrine / 視床下部 / ド-パミン / LHーRH / TNFーα / インタ-ロイキン6 / エストロジェン
Research Abstract

There is an accumulating evidence of a close linkage between neuroendocrine and immune system. Studies are designed to investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on hypothalamo-pituitary-ovarian axis. First, we demonstrated that TNF-alpha stimulated interleukin-6 (IL-6) production by hypothalamic cells. Further we have shown that IL-6 stimulated GnRH release from hypothalamic cessl(1), suggesting that cytokines such as IL-6 and TNF-alpha are involved in the feedback mechanism of estrus cycle. We also demonstrated that TNF-alpha stimulated IL-6 production by anterior pituitary cells and our results also suggested that folliculo-stellate (FS ) cell is one of the candidates of IL-6-producing cells(2). In addition, we observed that TNF-alpha stimulated the release of arachidonate from anterior pituitary cells and arachidonate metabolites such as 5-lipoxygenase products are involved in the process of prolactin release(3).

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Yamaguchi M.: "The interferon family stimulates the secretions of prolactin and interleukin-6 by the pituitary bland in vitro." J.Endocrinol.Invest.14. 457-461 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koike K.: "Tumor necrosis factor-α stimulates prolactin release from anterior pituitary cells:A possible involvement of intracellular calcium mobilization." Endocrinology. 128. 2785-2790 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koike K.: "Tumor necrosis factor-α increases release of arachidonate and prolactin from anterior pituitary cells." Endocrinology. 128. 2791-2798 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamaguchi M.: "Interleukin-6 stimulates gonadotropin-releasing hormone secretion from rat hypothalamic cells" Horm.Res.55. 199-203 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masumoto N.: "Pituitary follicuro-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pituitary adenylate cyclase-activating peptide),showing increased adenosine 3'5'-monophosphate and interleukin-6 secietion and cell proliferation." Endocrinology. 133. 2159-2155 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohmichi M.: "Binding sites for interleukin-6 in the anterior pituitary gland." Neuroendocrinology. 55. 199-203 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamaguchi M.et al.: "Interleukin-6 stimulates gonadotropin-releasing hormon secretion from rat hypothalamic cells" Horm.Res.35. 252-256 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koike K.et al.: "Tumor necrosis factor-alpha stimulates prolactin release from anterior pituitary cells : A possible involvement of intracellular calcium mobilization" Endocrinology. 128. 2785-2790 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koike K.et al.: "Tumor necrosis factor-alpha increases release of arachidonate and prolactin from anterior pituitary cells" Endocrinology. 128. 2791-2798 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Jikihara H.: "Intraventricular administration of Histidyl-Proline-Diketopiperazine[Cyclo(His-Pro)]suppresses prolactin secretion and synthesis:A possible role of[Cyclo(His-Pro)]as dopamine uptake blocker in rat hypothalamus" Endocrinology. 132. 953-958 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masumoto N.: "Pituitary folliculo-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pituitry adenylate cyclase-activating peptide),showing increased adenosine 3′5′-monophosphate and interleukin-6 secretion and cell proliferation" Endocrinology. 133. 2159-2155 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koike K.: "Prolactin stimulates 3H dopamine release from dispersed rat tuberoinfundibelar dopaminergic neurons and dopamine decreases gonadotropin-releasing honmone release induced by calcium ionophore" Acta Endocrinol.129. 548-553 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishikawa Y.: "Effect of tyrotropin-releasing hormone and phorbol ester on dopamine release from dispersed rat tuberoinfundibular dopaminergic neurons" Peptides. 14. 839-844 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohmichi M.: "Thyrotropin-releasing hormone stimulates MAP kinase activity in GH3 cells by divergent pathways.Evidence of a role for early tyrosine phosphorylation" J.Biol.Chem.

    • Related Report
      1993 Annual Research Report
  • [Publications] Kozo Kadowaki: "Atrial and brain natriuretic peptides enhance dopamine accumulation in cultured rat hypothalamic cells including dopaminergic neurons" Neuroendocrinology. 56. 11-17 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masahide Ohmichi: "Binding sites for interleukin-6 in the anterior pituitary gland" Neuroendocrinology. 55. 199-203 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kozo Kadowaki: "Vasoactive intestinal peptide enhances dopamine accumulation in primary cell culture of rat hypothalamus" Endocrinol Japon. 39. 197-202 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ikegami Hiromasa: "Intraventricular administration of thyrotrothyrotrophin-releasing hormone(TRH)suppresses prolactin secretion and synthesis:a possible involvement of dopamine release by TRH from rat hypothalamus" J Endocrinol. 133. 59-66 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masahide Ohmichi: "Immunohistochemical evidence that rat FSH cells contain β II-subspecies of protein kinase C" Endocrinol Japon. 39. 609-613 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Yamaguchi: "Shortーterm treatment with 17ーβ estradiol enhances spontaneous [^3H]dopamine release cultured rat tuberoinfundileular neurons" J.Endocrinol.Invest.14. 187-191 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Koike: "Tumor necrosis factorーα stimulates prolactin release from pitutary cells:A possible involvement of intracellular calcium mobilization" Endocrinology. 128. 2785-2790 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Koike: "Tumor necrosis factorーα increases release of arachidonate and prolactin from rat anterior pituitary cells" Endocrinology. 128. 2791-2798 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Koike: "Effect of prolactin on the secretion of hypothalamic GnRH and pituitary gonadotropins" Horm Res. 35. 5-12 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Yamaguchi: "Effect of TNFーα on prolactin seccetion from rat anterior pituitary and dopamine release from the hypothalamus:comparison with the effect of interleukinー1β" Endocrinol Japan. 38. 357-361 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Ikebuchi: "Superoxide anion increases intracellular pH,intracellular free calcium and arachidonate release in human amnion cells" J.Biol chem. 266. 13233-13237 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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