| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
We have a purpose to investigate the possibility of the allo-artificial mucosal transplantation using the frozen cells derived from human oral mucosa. First, we studied the surviving process of the auto-artificial mucosal transplantation. Oral mucosal cells were harvested from rabbit oral cavity and cultured. 10^6 of fibroblasts were cultured in the collagen gel for 7 days, and next on it, epithelial cells were cultured for more 3 days and artificial mucosa was compleated. Rabbit dorsal area was prepared as the recipient site and grafted artificial mucosa were observed on 1, 3, 5, 7, 10, 14, 21, 28 days after transplantation. Microscopic observation revealed the neovascularization process and it resulted that capillary infiltration began on 3 days after transplantation and the number of the capillaries were increased gradually day by day. On the 21st day, the vascular network like the living tissue was constructed. The epitheliarization was observed as the negative growth on the 3rd days because of lack of nutrial support for 3days just after transplantation. But epitheliarization was improved as neovascularization and epitheliall ayer was completed. On the 21st day, PAS positive structure and rete peg like structure were found just below the epithelial layer. Summarizing these results, we could get the conclusion that auto-artificial mucosal transplantation has the similar process to the usual auto-skin or mucosal graft and it suggests the possibility of clinical application.
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