| Project/Area Number |
03833004
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子細胞生物学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TASHIRO Kosuke The University of Tokyo, Faculty of Science, Lecturer, 理学部, 講師 (00192170)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOKAWA Koichir The University of Tokyo, Faculty of Science, Professor, 理学部, 教授 (20037295)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Hepatocyte Growth Factor / Gene Structure / induction of expression |
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| Research Abstract |
1) Structure of the rat hepatocyte growth factor gene
We isolated the rat genomic clones coding the whole region of HGF from rat genomic library. The rat HGF is encoded in about 70 kbp genomic region and divided into 18 exons. The complete nucleotide sequences of its promoter region and the flanking regions between each exons and introns were determined. In the promoter region, the TATA and GC box sequences are not observed so that the promoter of rat HGF may has unique characters. There are some consensus sequences of various enhancer, such as AP1 and Sp1 binding site, APRF, and NF-1. Especially, the existence of APRF elements suggest that the expression of HGF are regulated by cytokines.
By S1-protection and primer-extension analysis, it is found that there are at least four transcription start sites in HGF promoter region. When hepatitis was induced by CC14, the transcription from the 3rd start site (site B) increased dramatically, indicating that the transcriptional regulation from site B is an important for the expression of HGF in the injury.
2) Development of the assay systems for HGF expression
To establish the assay systems of HGF induction mechanisms, we try to culture the non-parenchymal cells. Until now, we have developed the method for the separation and the culture of the non-parenchymal livercells.
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