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Cytoskeletal proteins working in the process of secretion

Research Project

Project/Area Number 03833007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 分子細胞生物学
Research InstitutionThe University of Tokyo

Principal Investigator

MAEKAWA Shohei  The University of Tokyo, Faculty of Science, Assistant, 理学部, 助手 (40173695)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsSecretion / Neuron / Cytoskeleton
Research Abstract

1. Characterization of the cytoskeletal proteins of adrenal medulla. A new actin binding protein was found and purified from the membrane fraction of adrenal medulla. This protein was assumed to be a G-protein, for this protein was solubilized from the membrane fraction using GTP S, a nonhydrolyzable GTP analogue. Immunoblotting experiments using antibodies against known G proteins, such as Gi, Go, and Gs, however, showed no reactivity with this protein and antibody prepared against this protein showed also no reactivity with these G-proteins. A structural analysis of this protein is now under progress.
2. Analysis of membrane proteins of the central nervous system. A new protein, rich in the membrane fraction of brain tissue and having a calmodulin binding activity, was purified and its molecular structure was clarified by cDNA cloning. This protein was an acidic protein having a unique amino acids composition containing few hydrophobic residues and showed resistance to heat and acid treatments. These characters resemble to these of MARCKS and GAP-43. In its N-terminal end, this protein has a myrystoylation site and the addition of this lipid is thought to be responsible to its translocation to the cell membrane. Studies using specific antibody showed its specific expression in the nervous tissue, its developmentally changed expression and its localization in the membrane of the neuron.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] MAEKAWA,Shohei: "Puaification of a Membraueous Actin Binding Protein from Bovine Advehol Medulla" BIOCHEMISTRY INTERNATIONAL. 28. 87-95 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MAEKAWA, Shohei: "Purification of a Membraneous Actin Binding Protein from Bovine Adrenal Medulla." BIOCHEMISTY International. 28. 87-95 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MAEKAWA: "Purification of a membraneous Actin Binding Protein from bovine advenal medulla" BIOCHEMISTRY International. 28. 87-95 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Maekawa,S.: "Purification and partial characterization of a novel actin binding Protein of adrenal medulla."

    • Related Report
      1991 Annual Research Report
  • [Publications] Maekawa,S.: "Purification and molecular cloning of a novel calmodulin binding protein of rat brain."

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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