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Analysis of growth regulating factors produced by mesenchymal cells of the chick embryo in complete protein-free primary cultures.

Research Project

Project/Area Number 03833023
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 分子細胞生物学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

WATANABE Kazuo  Hiroshima Univ., Fac.of Integr.Arts Sci., Assoc.Prof., 総合科学部, 助教授 (00158619)

Co-Investigator(Kenkyū-buntansha) KAWAHARA Akira  Hiroshima Univ., Fac.of Integr.Arts Sci., Assoc.Prof., 総合科学部, 助教授 (50112157)
AMANO Minoru  Hiroshima Institute of Technology, Fac.of Environmental Sci., Prof., 環境学部, 教授 (00076986)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsprotein-free culture / chondrocyte / fibroblast / cartilage / mesenchyme / sclera / autocrine / growth factor / ニワトリ胚 / 胚細胞
Research Abstract

In the chick embryo, there is a population of chondrogenic fibroblasts known as scleral fibroblasts. Scleral fibroblasts in primary culture secrete multiple autocrine growth-promoting factors, scleral autocrine factors (SAFs), into protein-free medium. One such factor, SAF-IIa, which is heat-labile and binds to heparin, shows strong DNA synthesis-promoting activity on the mouse fibroblast cell line, BALB/c 3T3 A31 cells and has a molecular weight of ca. 16kDa by gel filtration. These data suggest that SAF-IIa is related to growth factors of the FGF family. However, the effects of heparin augumentation on the growth-promoting activity suggest that SAF-IIa is not identical to aFGF or bFGF, when assayd on scleral fibroblasts and also on BALB/c 3T3 A31 cells. The other heat-labile autocrine growth-promoting factor, SAF-IIb, shows weak binding to heparin and no growth-promoting activity for BALB/c 3T3 A31 cells. The heatresistant growth factor, SAF-I, is effective in enhancing the proliferation of BALB/c 3T3 cells and its activity is increased by heat treatment. Whole-embryo fibroblasts, which show low autocrine growth in protein-free medium, produce mainly SAF-IIa-like growth-promoting activity and do not produce SAF-I.This indicates that the strong proliferative activity of scleral fibroblasts in vitro can be attributed to the production of a strong and stable autocrine factor, SAF-I, in the growing phase and is a specialized property of the chondrogenic cells of the sclera.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kazuo Watanabe: "Analysis of autocrine growth regulators produced by chondrogenic fibrolasts of chick embryo sclera in protein-free primary cultures." Cell Proliferation. 27(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshie Ohya: "Control of growth and differentiation of chondrogenic fibroblasts in soft-agar culture:Role of basic fibroblast growth factor and transforming growth factor-β." Zoological Science. 11(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuo Watanabe: "Scleral fibroblasts of the chick embryo differentiate into chondrocytes in soft-agar culture." In Vitro Cellular and Developmental Biology. 28A. 603-608 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshie Ohya: "Scleral Fibroblasts of the chick embryo can proliferate without transferrin in protein-free culture." Zoological Science. 9. 749-755 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuo Watanabe, Tatsuya Miyazaki, Fumio Oiji and Toshihiko Tsuda: "Analysis of autocrine growth regulators produced by chondrogenic fibroblasts of chick embryo sclera in protein-free primary cultures." Cell Proliferation. 27, (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshie Ohya and Kazuo Watanabe: "Control of growth and differentiation of chondrogenic fibroblasts in soft-agar culture : Role of basic fibroblast growth factor and transforming growth factor-beta." Zoological Science. 11, (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuo Watanabe, Kumiko Yagi, Yoshie Ohya and Koji Kimata: "Scleral fibroblasts of the chick embryo differentiate into chondrocytes in soft-agar culture." In Vitro Cellular and Developmental Biology. 28A. 603-608 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yoshie Ohya, Kazuo Watanabe, Nobuo Shimamoto and Minoru Amano: "Scleral Fibroblasts of the chick embryo can proliferate without transferrin in protein-free culture." Zoological Science. 9. 749-755 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Ohya: "Scleral fibroblasts of the chick embryo can proliferate without transferrin in protein-free culture." Zoological Science. 9. 749-755 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Watanabe: "Scleral fibroblasts of the chick embryo differentiate into chondrocytes in soft-agar culture." In Vitro Cellular and Developmental Biology. 28A. 603-608 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Ohya, K.Watanabe N.Shimamoto, M.Amano: "Scleral fibroblasts of the chick embryo can proliferate without transferrin in protein‐free culture." Zoological Science. 9. 749-755 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Watanabe, K.Yagi,Y.Ohya, K.Kimata: "Scleral fibroblasts of the chick embryo differentiate into chondrocytes in soft‐agar culture." In Vitro Cellular and Developmental Biology. 28A. 603-608 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Watanabe K.,K.Yagi,Y.Ohya and K.Kimata: "Scleral fibroblasts of the chick embryo differentiate into chondrocytes in softーagar culture." In Vitro Cellular & Developmental Biology. 28A. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ohya Y.and K.Watanabe: "Transferrin is not required for growth of scleral fibroblasts in proteinーfree culture." Zoological Science. 8. 1101-1101 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Morita R.and K.Watanabe: "Effects of retinoic acid on proliferation of precartilage cells of the chick embryo in proteinーfree culture." Cell Structure and Function. 16. 589-589 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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