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Molecular Cytogenetical Studies on the Avian Sex Chromosomes

Research Project

Project/Area Number 04044028
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTohoku University

Principal Investigator

MIZUNO Shigeki  Faculty of Agriculture, Tohoku University, 農学部, 教授 (90112903)

Co-Investigator(Kenkyū-buntansha) HARATA Masahiko  Faculty of Agriculture, Tohoku University, 農学部, 助手 (70218642)
HUTCHISON Nancy  Fred Hutchinson Cancer Research Center (U.S.A.), スタッフサイエンテイ
NANCY Hutchi  フレッドハッチソンがん研究センター (アメリカ), スタッフサイエンテイ
NANCY J Hutc  フレッドハッチンソンがん研究センター, (アメリカ、シアトル), スタッフサイエンティ
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1994: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1993: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsChicken / Sex Chromosomes / Z Chromosome / W Chromosome / Gene Mapping / Heterochromatin / Lampbrush Chromosome / Fluorescence In Situ Hybridization (FISH) / 減数分裂期染色体 / ZW染色体 / 卵母細胞 / 減数分裂 / 2価染色体 / ゲノムマッピング
Research Abstract

The aim of this joint research was to isolate clones from the chicken W and Z chromosome-specific genomic libraries, determine their nucleotide sequences, and to examine their localizations on the lampbrush Z-W bivalent chromosomes by fluorescence in situ hybridization (FISH). The major outcome is as follows.
1) Z chromosome : Lambda gt10 libraries were prepared from a terminal region of the chicken Z chromosome by utilizing argon ion laser micro-irradiation to burn down all other chromosomes, micro-extraction of DNA from the remaining chromosomal region and random PCR amplification using a single unique primer. One hundred clones were selected randomly, mixed and used as probes in the FISH to mitotic chromosomes. Those probes hybridized to the heterochromatic end region of the chicken Z chromosome. A lambda GEM12 genomic library of the female chicken was screened with those mixed probes and a clone, pCHZTH8 was obtained. This clone hybridized to the short loops region and the terminal bow-like loop (TBL) at the heterochromatic end of the Z chromosome. A part of the pCHZTH8 sequence is conserved among the order Galliformes but most of them is unique to the genus Gallus.
2) W chromosome : The same technique as above was applied to obtain W chromosome-specific genomic libraries. Two regions on the chicken W chromosome, CW01 and CW50 each consisting of about 25-kb non-repetitive sequences, were cloned. The CW50 region contains a putative CpG island and the CW01 region contains a sequnce that is conserved widely as W-linked among many avian species, and thus is a useful general probe for sexing avian species.

Report

(3 results)
  • 1994 Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Noriyuki Suka: "W-heterochromatin of chicken; its unusual DNA components, late replication, and chromatin structure" Genetica. 88. 93-105 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasushi Saitoh: "Identification and localization of two genes on the chicken Z chromosome: implication of evolutionary conservation of the Z chromosome among avian species" Chromosome Res.1. 239-251 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noriyuki Suka: "W-heterochromatin of chicken ; its unusual DNA components, late replication, and chromatin structure" Genetica. 88. 93-105 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasushi Saitoh: "Identification and localization of two genes on the chicken Z chromosome : implication of evolutionary conservation of the Z chromosome among avian species" Chromosome Res.1. 239-251 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noriyuki Suka: "W-heterochromatin of chicken;its unusual DNA components,late replication,and chromatin structure" Genetica. 88. 93-105 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasushi Saitoh: "Identification and localization of two genes on the chicken Z chromosome:implication of evolutionary conservation of the Z chromosome among avian species" Chromosome Res.1. 239-251 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.SAITOH: "Distibution of XhoI and EcoRI fanily repetitive DNA sequences into separete domains in the chicken W chromosome" Chromosoma. 101. 474-477 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.MIZUNO: "Sex-specific DNA sequences in Galliformes and their application to the study of sex differentiation In:Manipulation of the Avian Genome(R.J.Etches and A.M.V.Gibbins,eds.)" CRC Press,Boca Raton,Florida,U.S.A., 322 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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