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Cytogenetic Studies on the Plasmacytomagenesis in SCID mice

Research Project

Project/Area Number 04044073
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionCancer Research Institute Kanazawa University

Principal Investigator

OHNO Shinsuke  Cancer Research Institute, Kanazawa University, がん研究所, 助教授 (70019868)

Co-Investigator(Kenkyū-buntansha) WIENER Francis  Department of Tumorbiology, Karolinska Institute, 腫瘍生物部, 教授
MATSUSHIMA Kouji  Cancer Research Institute, Kanazawa University, がん研究所, 教授 (50222427)
MURAKAMI Seishi  Cancer Research Institute, Kanazawa University, がん研究所, 教授 (90019878)
HAYAKAWA Jun-ichiro  School of Medicine, Kanazawa University, 医学部, 教授 (50110622)
FRANCIS Wien  カロリンスカ研究所, 腫瘍生物部, 教授
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 1994: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1992: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsSCID mouse / Plasmacytoma / Cytogenetics / Chromosomal translocation / Abelson leukemia virus / Abelson白血病ウィルス
Research Abstract

In order to determine the timing of the chromosomal translocations specific for murine plasmacytomas (t (12 ; 15) or t (6 ; 15) ) with respect to the stages of B-cell development, C.B-17 scid/scid mice were reconstituted with SRBC-immunized or normal BALB/c6.15 mouse spleen and bone marrow cells. Plasmacytomas were induced by i.p.injection of pristane alone or of pristane + Abelson murine leukemia virus (A-MuLV).
Five plasmacytomas developed in 19 SCID mice. Karyotype analysis showed that 3 were of donor (BALB/c6.15) origin and 2 were of host (C.B-17scid/scid) origin. Plasmacytomas originated in SCID mice (ABPC-SCID-IM-B and IM-D) had a・ t (6 ; 15) chromosomal translocation. Precursor cells from which the plasmacaytomas carrying t (6 ; 15) chromosomal translocation developed might be in a pro-, pre-B cell stage, because no functional B-cells develop in SCID mice by the defect of V- (D) -J recombination system. Ig allotype of C.B-17scid/scid mouse is "b". However, both IM-B and IM-D were … More IgA-producers of "a" allotype. This indicates that IM-B and IM-D did not develop from "leaky" SCID cells. SSLP (Simple Sequarance Repeat Length Polymorphism) analysis using several microsatellite DNAs of known chromosomal locations suggested that the Igh-C regions of chromosome 12 of IM-B and IM-D were replaced by those of chromosome 12 of BALB/c6.15 cells, therefore synthesizing IgA of "a" allotype.
In the next series of experiments, C.B-17scid/scid mice were reconstituted with surface Ig-positive cells (immature and mature B) obtained from BALB/c6.15 mouse spleens. Plasmacytomas were induced as mentioned above. Six plasmacytomas were induced by pristane + A-MuLV in 80 SCID mice. Four plasmacytomas developed by pristane alone in 40 SCID mice, so far. Karyotype analysis revealed that all the plasmacytomas developed were of donor mouse (BALB/c6.15) cell origin. Interestingly, all the plasmacytomas developed so far carried t (12 ; Rb (6.del 15) ) chromosomal translocation irrespective of the inducing agents. Therefore, precursor cells from which the plasmacytomas carrying a t (12 ; 15) chromosomal translocation develop are suggested to be sIg-positive, immature and/or mature B cells. Less

Report

(3 results)
  • 1994 Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shinsuke OHNO et al.: "Development of plasmacytomas in C.B-17scid/scid mice reconstituted with BALB/c6.15 mouse cells" Cancer Research Institute Report. 22-23 (1991-1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sachiko SUEMATSU et al.: "Generation of plasmacytomas with the chromosomal translocation t(12;15)in IL-6 transgenic mice" Proc.Natl.Acad.Sci.USA. 89. 232-235 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Francis WIENER et al.: "Functional homology between N-myc and c-myc in murine plasmacytomagenesis" Oncogene. 7. 1241-1249 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Francis WIENER et al.: "Plasmacytomagenesis in mice:Model for neoplastic development upon chromosomal translocation" Carcinogenesis. 12. 1681-1697 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大野眞介: "SCIDマウス" キーワード癌`95. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Francis WIENER et al.: "Non-random chromosomal change (trisomy 11)in murine plasmacytomas induced by an ABL-MYC retrovirus" Cancer Research. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shinsuke OHNO et al.: "Development of plasmacytomas in C.B-17scid/scid mice reconstituted with BALB/c6.15 mouse cells" Cancer Research Institute Report. 22-23 (1991-1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sachiko SUEMATSU et al.: "Generation of plasmacytomas with the chromosomal translocation t (12 ; 15) in IL-6 transgenic mice" Proc.Natl.Acad.Sci.USA. 89. 232-235 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Francis WIENER et al.: "Functional homology between N-myc and c-myc in murine plasmacytomagenesis" Oncogene. 7. 1241-1249 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Francis WIENER et al.: "Plasmacytomagenesis in mice : Model for neoplastic development upon chromosomal translocation" Carcinogenesis. 12. 1681-1697 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Seishi MURAKAMI et al.: "Transactivation of Human Hepatitis B virus X protein, HBx, is operated through the mechanism distinct from the protein kinase C and Okadaic acid activation pathways.Virology" 199. 243-246 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Francis WIENER et al.: "Non-random chromosomal change (trisomy 11) in murine plasmacytomas induced by ABL-MYC retrovirus" Cancer Research. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Wiener,S.Ohno,J.Hayakawa: "ABL-MYC-retrovirus-induced plasma-cytomagenesis in SCID mice" Cur.Top.Microbiol.Immunol.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] K.ウイルスなし:

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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